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published: 2021-04-11
This dataset contains RNASim1000, Cox1-Het datasets as well as analyses of RNASim1000, Cox1-Het, and 1000M1(HF).
keywords: phylogeny estimation; maximum likelihood; RAxML; IQ-TREE; FastTree; cox1; heterotachy; disjoint tree mergers; Tree of Life
planned publication date: 2021-05-07
- The research objectives were to evaluate the effect of dietary supplementation of white (WSH) and red (RSH) sorghum grains on gastrointestinal health of felines through the determination of apparent total tract macronutrient digestibility (ATTD), fecal characteristics, fermentative end-products, and microbiota, compared with a traditional corn-based diet (Control). <br />- Nine, intact, male domestic shorthaired cats (average age: 0.8 ± 0.00 yr; average weight: 4.5 ± 0.23 kg) were used in a triplicated 3 × 3 Latin square design. Total DNA from fresh fecal samples was extracted using Mo-Bio PowerSoil kits (MO BIO Laboratories, Inc., Carlsbad, CA). Amplification of the 292 bp-fragment of V4 region from the 16S rRNA gene was completed using a Fluidigm Access Array (Fluidigm Corporation, South San Francisco, CA). Paired-end Illumina sequencing was performed on a MiSeq using v3 reagents (Illumina Inc., San Diego, CA) at the Roy J. Carver Biotechnology Center at the University of Illinois. <br />- Filenames are composed of animal name identifier, supplement (C=control; RS=red sorghum grain; WS= white sorghum grain) and period replicate number (P1, P2 and P3).
keywords: carbohydrate; felines; gut health; microbiota; sorghum; nutrient digestibility;, ancient grain
planned publication date: 2021-05-07
- The objective of this study was to evaluate macronutrient apparent total tract digestibility (ATTD), gastrointestinal tolerance, and fermentative end-products in extruded, canine diets. <br />- Five diets were formulated to be isocaloric and isonitrogenous with either garbanzo beans (GBD), green lentils (GLD), peanut flour (PFD), dried yeast (DYD), or poultry by-product meal (CON) as the primary protein sources. Ten adult, intact, female beagles (mean age: 4.2 ± 1.1 yr, mean 28 weight: 11.9 ± 1.3 kg) were used in a replicated, 5x5 Latin square design with 14 d periods. Total DNA from fresh fecal samples was extracted using Mo-Bio PowerSoil kits (MO BIO Laboratories, Inc., Carlsbad, CA). Amplification of the 292 bp-fragment of V4 region from the 16S rRNA gene was completed using a Fluidigm Access Array (Fluidigm Corporation, South San Francisco, CA). Paired-end Illumina sequencing was performed on a MiSeq using v3 reagents (Illumina Inc., San Diego, CA) at the Roy J. Carver Biotechnology Center at the University of Illinois. <br />- Filenames are composed of animal name identifier, diet (CON=control; DY= dried yeast; GB= garbanzo beans; GL= green lentils; PF= peanut flour) and period replicate number (P1, P2, P3, P4, and P5).
keywords: Dog; Digestibility; Legume; Microbiota; Pulse; Yeast
planned publication date: 2021-05-14
- The aim of this research was to evaluate the novel dietary fiber source, miscanthus grass, in comparison to traditional fiber sources, and their effects on the microbiota of healthy adult cats. Four dietary treatments, cellulose (CO), miscanthus grass fiber (MF), a blend of miscanthus fiber and tomato pomace (MF+TP), or beet pulp (BP) were evaluated.<br /><br />- The study was conducted using a completely randomized design with twenty-eight neutered adult, domesticated shorthair cats (19 females and 9 males, mean age 2.2 ± 0.03 yr; mean body weight 4.6 ± 0.7 kg, mean body condition score 5.6 ± 0.6). Total DNA from fresh fecal samples was extracted using Mo-Bio PowerSoil kits (MO BIO Laboratories, Inc., Carlsbad, CA). Amplification of the 292 bp-fragment of V4 region from the 16S rRNA gene was completed using a Fluidigm Access Array (Fluidigm Corporation, South San Francisco, CA). Paired-end Illumina sequencing was performed on a MiSeq using v3 reagents (Illumina Inc., San Diego, CA) at the Roy J. Carver Biotechnology Center at the University of Illinois. <br />- Filenames are composed of animal name identifier, diet (BP= beet pulp; CO= cellulose; MF= miscanthus grass fiber; TP= blend of miscanthus fiber and tomato pomace).
keywords: cats; dietary fiber; fecal microbiota; miscanthus grass; nutrient digestibility; postbiotics
published: 2020-11-25
Video recorded by Louise Barker using a Cannon Powershot camera documents late-season combat behavior in Agkistrodon contortrix. Recorded in Beaufort County, North Carolina, 11.1 km SE of downtown Washington on 21 October 2020.
keywords: Agkistrodon contortrix; combat; mating; reproduction; copperhead; pit viper; Viperidae;
published: 2020-12-14
Femoral skeletal traits (cross-sectional properties, maximum distal metaphyseal breadth of the femur, and maximum superior/inferior femoral head diameter) of 219 Taiwanese subadult individuals (aged 0 to 17) as used in the manuscript "Allometric scaling and growth: evaluation and applications in subadult body mass estimation."
keywords: femur; cross-sectional geometry; osteometrics; subadult
published: 2020-12-29
Three datasets: species_abundance_data, species_traits, and environmental_data. The three datasets were collected in the Fortuna Forest Reserve (8°45′ N, 82°15′ W) and Palo Seco Protected Forest (8°45′ N, 82°13′ W) located in western Panama. The two reserves support humid to super-humid rainforests, according to Holdridge (1947). The species_abundance_data and species_traits datasets were collected across 15 subplots of 25 m2 in 12 one-hectare permanent plots distributed across the two reserves. The subplots were spaced 20 m apart along three 5 m wide transects, each 30 m apart. Please read Prada et al. (2017) for details on the environmental characteristics of the study area. Prada CM, Morris A, Andersen KM, et al (2017) Soils and rainfall drive landscape-scale changes in the diversity and functional composition of tree communities in a premontane tropical forest. J Veg Sci 28:859–870. https://doi.org/10.1111/jvs.12540
keywords: functional traits; plants; ferns; environmental data; Fortuna; species data; community ecology
published: 2021-01-23
Data sets from "Comparing Methods for Species Tree Estimation With Gene Duplication and Loss." It contains data simulated with gene duplication and loss under a variety of different conditions.
keywords: gene duplication and loss; species-tree inference;
published: 2021-02-15
The file contains biomass and count data of food items encountered in the digestive tract of collected green-winged teal from the Illinois River Valley during spring 2016-2018. The file also contains biomass of food items collected from core samples collected at sites where the green-winged teal were collected. Together, the consumed and availability food data are used to calculate diet selection. The data also contains information on the teal, collection, sites, and other covariates used in analysis. Lastly, the dataset contains biomass of food items collected in medium (#35) and small (#60) sieves for 2018 core samples.
keywords: Anas crecca; food selection; green-winged teal; Illinois River Valley; moist-soil plants; spring migration; stopover ecology
published: 2021-02-25
Total nitrogen leaching rates were calculated over the Mississippi Atchafalaya River Basin (MARB) using an integrated economic-biophysical modeling approach. Land allocation for corn production and total nitrogen application rates were calculated for crop reporting districts using the Biofuel and Environmental Policy Analysis Model (BEPAM) for 5 RFS2 policy scenarios. These were used as input in the Integrated BIosphere Simulator-Agricultural Version (Agro-IBIS) and the Terrestrial Hydrologic Model with Biogeochemistry (THMB) to calculate the nitrogen loss. Land allocation and total nitrogen application simulations were simulated for the period 2016-2030 for 303 crop reporting districts (https://www.nass.usda.gov/Data_and_Statistics/County_Data_Files/Frequently_Asked_Questions/county_list.txt). The final 2030 values are reported here. Both are stored in csv files. Units for land allocation are million ha and nitrogen application are million kg. The nitrogen leaching rates were modeled with a spatial resolution of 5' x 5' using the North American Datum of 1983 projection and stored in NetCDF files. The 30-year average is calculated over the last 30 years of the 45 years being simulated. Leaching rates are calculated in kg-N/ha.
keywords: nitrogen leaching, bioethanol, bioenergy crops
published: 2021-03-05
Adey_Larson_Behavior.csv: Results of behavioral assays for rusty crayfish Faxonius rusticus collected from six lakes in Vilas County, Wisconsin in summer 2018. Crayfish_ID is an individual crayfish ID or identifier that matches to individuals in Adey_Larson_Isotope. Collection is how organisms were collected (trapped = baited trapping, snorkel = by hand). Lake is the study lake crayfish were collected from. Length is crayfish carapace length in mm. CPUE is crayfish catch-per-unit effort from baited trapping in that lake during summer 2018. Shelter_Occupancy, Exploration, Feeding_Snail, Feeding_Detritus, Feeding_Crayfish, and Aggressiveness are behavioral assay scores for individual crayfish. Shelter_Occupancy is frequency of observation intervals (12 maximum) in which crayfish were observed in shelter over a 12 hour period. Exploration is time for crayfish to explore a new area measured in seconds (maximum possible time 1200 seconds or 20 minutes). Feeding_Snail, Feeding_Detritus, and Feeding_Crayfish is the time for crayfish to take a food item (snail, detritus, or snail in the presence of another crayfish) measured in seconds (maximum possibe time 1200 seconds or 20 minutes). Aggressiveness is the response to an approach with a novel object scored as a fast retreat (-2), slow retreat (-1), no visible response (0), approach without threat display (1), approach with threat display (2), interaction with closed chelae (3), or interaction with open chelae (4). Three repeated aggressiveness measures were made per individual (Aggresiveness1, Aggresiveness2, Aggresiveness3), which were summed for inclusion in subsequent analyses (Aggresiveness_Sum). More detailed behavioral assay methods can be found in Adey 2019 Masters thesis. Adey_Larson_Isotope.csv: Stable isotope (13C, 15N) values for rusty crayfish Faxonius rusticus and snail or mussel primary consumers from six lakes in Vilas County, Wisconsin collected during summer 2018. Crayf is an individual crayfish ID or identifier that matches to the same individual crayfish in Adey_Larson_Behavior. Lake is the study lake. Collection is how organisms were collected (trapped = baited trapping, snorkel = by hand). Sample type indicates whether isotope values are for crayfish, snail, or mussel. d13C and d15N are stable isotope values.
keywords: individual specialization; intraspecific competition; behavior; diet; stable isotopes; crayfish; invasive species; limnology; Faxonius rusticus
published: 2021-03-05
Datasets that accompany Beilke, Blakey, and O'Keefe 2021 publication (Title: Bats partition activity in space and time in a large, heterogeneous landscape; Journal: Ecology and Evolution).
keywords: spatiotemporal; chiroptera
published: 2021-03-15
Dataset associated with "Hiding in plain sight: genetic confirmation of putative Louisiana Fatmucket Lampsilis hydiana in Illinois" as submitted to Freshwater Mollusk Biology and Conservation by Stodola et al. Images are from cataloged specimens from the Illinois Natural History Survey (INHS) Mollusk Collection in Champaign, Illinois that were used for genetic research. File names indicate the species as confirmed in Stodola et al. (i.e., Lampsilis siliquoidea or Lampsilis hydiana) followed by the INHS Mollusk Collection catalog number, followed by the individual specimen number, followed by shell view (interior or exterior). If no specimen number is noted in the file name, there is only one specimen for that catalog number. For example: Lsiliquoidea_46515_1_2_3_exterior. Images were created by photographing specimens on a metric grid in an OrTech Photo-e-Box Plus with a Nikon D610 single lens reflex camera using a 60mm lens. Post-processing of images (cropping, image rotation, and auto contrast) occurred in Adobe Photoshop and saved as TIFF files using no image compression, interleaved pixel order, and IBM PC Byte Order. One additional partial lot, INHS Mollusk Catalog No. 37059 (shown with both interior and exterior view in one image), is included for reference but was not genetically sequenced. A .csv file contains an index of all specimens photographed. SPECIES: species confirmed using genetic analyses GENE: cox1 or nad1 mitochondrial gene ACCESSION: GenBank accession number INHS CATALOG NO: Illinois Natural History Survey Mollusk Collection Catalog number WATERBODY: waterbody where specimen was collected PUTATIVE SPECIES: species determination based on morphological characters prior to genetic analysis Phylogenetic sequence data (.nex files) were aligned using BioEdit (Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41:95-98.). Pertinent methodology for the analysis are contained within the manuscript submittal for Stodola et al. to Freshwater Mollusk Biology and Conservation. In these files, "N" is a standard symbol for an unknown base.
keywords: Lampsilis hydiana; Lampsilis siliquoidea; unionid; Louisiana Fatmucket; Fatmucket; genetic confirmation
planned publication date: 2022-01-01
The file “Fla.fasta”, comprising 10526 positions, is the concatenated amino acid alignments of 51 orthologues of 182 bacterial strains. It was used for the maximum likelihood and maximum parsimony analyses of Flavobacteriales. Bacterial species names and strains were used as the sequence names, host names of insect endosymbionts were shown in brackets. The file “16S.fasta” is the alignment of 233 bacterial 16S rRNA sequences. It contains 1455 positions and was used for the maximum likelihood analysis of flavobacterial insect endosymbionts. The names of endosymbiont strains were replaced by the name of their hosts. In addition to the species names, National Center for Biotechnology Information (NCBI) accession numbers were also indicated in the sequence names (e.g., sequence “Cicadellidae_Deltocephalinae_Macrostelini_Macrosteles_striifrons_AB795320” is the 16S rRNA of Macrosteles striifrons (Cicadellidae: Deltocephalinae: Macrostelini) with a NCBI accession number AB795320). The file “Sulcia_pep.fasta” is the concatenated amino acid alignments of 131 orthologues of “Candidatus Sulcia muelleri” (Sulcia). It contains 41970 positions and presents 101 Sulcia strains and 3 Blattabacterium strains. This file was used for the maximum likelihood analysis of Sulcia. The file “Sulcia_nucleotide.fasta” is the concatenated nucleotide alignment corresponding to the sequences in “Sulcia_pep.fasta” but also comprises the alignment of 16S rRNA. It has 127339 positions and was used for the maximum likelihood and maximum parsimony analyses of Sulcia. Individual gene alignments (16S rRNA and 131 orthologues of Sulcia and Blattabacterium) are deposited in the compressed file “individual_gene_alignments.zip”, which were used to construct gene trees for multispecies coalescent analysis. The names of Sulcia strains were replaced by the name of their hosts in “Sulcia_pep.fasta”, “Sulcia_nucleotide.fasta” and the files in “individual_gene_alignments.zip”. In all the alignment files, gaps are indicated by “-”.
keywords: endosymbiont, “Candidatus Sulcia muelleri”, Auchenorrhyncha, coevolution
planned publication date: 2021-11-16
Data from an a field experiment at El Velo, Chiriqui, Republic of Panama. Data contain information about functional traits of seedlings growing in different treatments including type of forest, nitrogen addition and organic matter.
keywords: Mycorrhiza; nitrogen; oak forest; Panama; plant-soil feedbacks, seedling growth
published: 2021-02-26
These data were used in the survival and cause-specific mortality analyses of translocated nuisance American black bear in Wisconsin published in Animal Conservation (Bauder, J.M., N.M. Roberts, D. Ruid, B. Kohn, and M.L. Allen. Accepted. Lower survival of nuisance American black bears (Ursus americanus) is not due to translocation. Animal Conservation). Included are CSV files including each bear's capture history and associated covariates and meta-data for each CSV file. Also included is an example R script of how to conduct the analyses (this R script is also included as supporting information with the published paper).
keywords: black bear; survival; translocation; nuisance wildlife management
published: 2021-03-22
R codes to implement data processing and econometric analysis in the accepted journal article: B Chen, BM Gramig and SD Yun. “Conservation Tillage Mitigates Drought Induced Soybean Yield Losses in the US Corn Belt.” Q Open.
keywords: R, Conservation Tillage, Drought, Yield, Corn, Soybeans, Resilience, Climate Change
published: 2020-10-20
This dataset includes a total of 501 images of 42 fossil specimens of Striatopollis and 459 specimens of 45 extant species of the tribe Amherstieae-Fabaceae. These images were taken using Airyscan confocal superresolution microscopy at 630X magnification (63x/NA 1.4 oil DIC). The images are in the CZI file format. They can be opened using Zeiss propriety software (Zen, Zen lite) or in ImageJ. More information on how to open CZI files can be found here: [https://www.zeiss.com/microscopy/us/products/microscope-software/zen/czi.html#microscope---image-data].
keywords: Striatopollis catatumbus; superresolution microscopy; Cenozoic; tropics; Zeiss; CZI; striate pollen.
published: 2021-03-10
The PhytoplasmasRef_Trivellone_etal.fas fasta file contains the original final sequence alignment used in the phylogenetic analyses of Trivellone et al. (Ecology and Evolution, in review). The 27 sequences (21 phytoplasma reference strains and 6 phytoplasmas strains from the present study) were aligned using the Muscle algorithm as implemented in MEGA 7.0 with default settings. The final dataset contains 952 positions of the F2n/R2 fragment of the 16S rRNA gene. The data analyses are further described in the cited original paper.
keywords: Hemiptera; Cicadellidae; Mollicutes; Phytoplasma; biorepository
published: 2021-03-08
These are abundance dynamics data and simulations for the paper "Higher-order interaction between species inhibits bacterial invasion of a phototroph-predator microbial community". In this V2, data were converted in Python, in addition to MATLAB and more information on how to work with the data was included in the Readme.
keywords: Microbial community; Higher order interaction; Invasion; Algae; Bacteria; Ciliate
published: 2021-03-08
In a set of field studies across four years, the effect of self-shading on photosynthetic performance in lower canopy sorghum leaves was studied at sites in Champaign County, IL. Photosynthetic parameters in upper and lower canopy leaves, carbon assimilation, electron transport, stomatal conductance, and activity of three C4-specific photosynthetic enzymes, were compared within a genetically diverse range of accessions varying widely in canopy architecture and thereby in the degree of self-shading. Accessions with erect leaves and high light transmission through the canopy are henceforth referred to as ‘erectophile’ and those with low leaf erectness, ‘planophile’. In the final year of the study, bundle sheath leakiness in erectophile and planophile accessions was also compared.
keywords: Sorghum; Photosynethic Performance; Leaf Inclination
published: 2021-02-16
Data from census of peer-reviewed papers discussing nosZ and published from 2013 to 2019. These data were reported in the manuscript titled, "Beyond denitrification: the role of microbial diversity in controlling nitrous oxide reduction and soil nitrous oxide emissions" published in Global Change Biology as an Invited Report.
keywords: atypical nosZ; Clade II nosZ; denitrification; nitrous oxide; N2O reduction; non-denitrifier; nosZ; nosZ-II; nosZ Clade II; soil N2O emissions
published: 2020-12-30
High-speed X-ray videos of four E. abruptus specimens recorded at the Advanced Photron Source (Argonne National lab) in the Summer of 2018 and corresponding position data of landmarks tracked during the motion. See readme file for more details.
published: 2020-10-01
We measured the effects of fire or drought treatment on plant, microbial and biogeochemical responses in temperate deciduous forests invaded by the annual grass Microstegium vimineum with a history of either frequent fire or fire exclusion. Please note, on Documentation tab / Experimental or Sampling Design, “15 (XVI)” should be “16 (XVI)”.
keywords: plant-soil interaction; grass-fire cycle; Microstegium; carbon and nitrogen cycling; microbial decomposers