Illinois Data Bank

Data for: Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification

We have recently created an approach for high throughput single cell measurements using matrix assisted laser desorption / ionization mass spectrometry (MALDI MS) (J Am Soc Mass Spectrom. 2017, 28, 1919-1928. doi: 10.1007/s13361-017-1704-1. Chemphyschem. 2018, 19, 1180-1191. doi: 10.1002/cphc.201701364). While chemical detail is obtained on individual cells, it has not been possible to correlate the chemical information with canonical cell types.
Now we combine high-throughput single cell mass spectrometry with immunocytochemistry to determine lipid profiles of two known cell types, astrocytes and neurons from the rodent brain, with the work appearing as “Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification” (DOI: 10.1002/anie.201812892).
Here we provide the data collected for this study. The dataset provides the raw data and script files for the rodent cerebral cells described in the manuscript.

Life Sciences
Single cell analysis; mass spectrometry; astrocyte; neuron; lipid analysis
CC BY
U.S. National Institutes of Health (NIH)-Grant:1U01MH109062
U.S. National Institutes of Health (NIH)-Grant:P30DA018310.
Jonathan Sweedler
2089 times
Version DOI Comment Publication Date
1 10.13012/B2-3125702_V1 2019-02-26

2.36 KB File
13.8 GB File
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986 MB File

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RelatedMaterial update: {"citation"=>["T. D. Do, J. F. Ellis, E. K. Neumann, T. J. Comi, E. G. Tillmaand, A. E. Lenhart, S. S. Rubakhin, J. V. Sweedler, ChemPhysChem 2018, 19, 1180.", "Do, TD, Ellis, JF, Neumann, EK, Comi, TJ, Tillmaand, EG, Lenhart, AE, Rubakhin, SS & Sweedler, JV (2018), Optically Guided Single Cell Mass Spectrometry of Rat Dorsal Root Ganglia to Profile Lipids, Peptides and Proteins. Chemphyschem: A European Journal Of Chemical Physics And Physical Chemistry, 19(10), 1180–1191. doi:10.1002/cphc.201701364"]} 2019-02-28T15:51:45Z
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RelatedMaterial update: {"material_type"=>["Article", "Article (International version)"], "selected_type"=>["Article", "Other"], "citation"=>["Neumann, E. K., Comi, T. J., Rubakhin, S. S., and Sweedler, J. V.: Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification, Angewandte Chemie., 2019, accepted. DOI: 10.1002/anie.201812892", "Neumann, E. ., Comi, T. ., Rubakhin, S. . and Sweedler, J. (2019), Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification. Angew. Chem. Int. Ed.. Accepted Author Manuscript. doi:10.1002/anie.201812892"]} 2019-02-28T15:51:45Z
Dataset update: {"subject"=>["", "Life Sciences"]} 2019-02-28T15:51:45Z
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