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Related
Article
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Do, TD, Ellis, JF, Neumann, EK, Comi, TJ, Tillmaand, EG, Lenhart, AE, Rubakhin, SS & Sweedler, JV (2018), Optically Guided Single Cell Mass Spectrometry of Rat Dorsal Root Ganglia to Profile Lipids, Peptides and Proteins. Chemphyschem: A European Journal Of Chemical Physics And Physical Chemistry, 19(10), 1180–1191. doi:10.1002/cphc.201701364
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Related
Article
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Comi T, Neumann E, Do T, Sweedler J. (2017), microMS: A Python Platform for Image-Guided Mass Spectrometry Profiling. Journal of the American Society for Mass Spectrometry, 28(9), 1919-1928. doi:10.1007/s13361-017-1704-1
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Related
Article (International version)
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Neumann, E. ., Comi, T. ., Rubakhin, S. . and Sweedler, J. (2019), Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification. Angew. Chem. Int. Ed.. Accepted Author Manuscript. doi:10.1002/anie.201812892
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Related
Article (German version)
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Neumann, E. ., Comi, T. ., Rubakhin, S. . and Sweedler, J. (2019), Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification. Angew. Chem.. Accepted Author Manuscript. doi:10.1002/ange.201812892
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Related
Article
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Neumann, E. , Ellis, F., Triplett, A., Rubakhin, S., and Sweedler, J. (2019), Lipid Analysis of Thirty-thousand Individual Rodent Cerebral Cells using High-Resolution Mass Spectrometry. Analytical Chemistry Just Accepted Manuscript. doi: 10.1021/acs.analchem.9b01689
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Dataset Description
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We have recently created an approach for high throughput single cell measurements using matrix assisted laser desorption / ionization mass spectrometry (MALDI MS) (J Am Soc Mass Spectrom. 2017, 28, 1919-1928. doi: 10.1007/s13361-017-1704-1. Chemphyschem. 2018, 19, 1180-1191. doi: 10.1002/cphc.201701364). While chemical detail is obtained on individual cells, it has not been possible to correlate the chemical information with canonical cell types.
Now we combine high-throughput single cell mass spectrometry with immunocytochemistry to determine lipid profiles of two known cell types, astrocytes and neurons from the rodent brain, with the work appearing as “Lipid heterogeneity between astrocytes and neurons revealed with single cell MALDI MS supervised by immunocytochemical classification” (DOI: 10.1002/anie.201812892).
Here we provide the data collected for this study. The dataset provides the raw data and script files for the rodent cerebral cells described in the manuscript.
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Subject
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Life Sciences
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Keywords
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Single cell analysis; mass spectrometry; astrocyte; neuron; lipid analysis
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License
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CC BY
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Funder
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U.S. National Institutes of Health (NIH)
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Grant:
1U01MH109062
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Funder
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U.S. National Institutes of Health (NIH)
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Grant:
P30DA018310.
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Corresponding Creator
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Jonathan Sweedler
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