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Illinois Data Bank Dataset Search Results
Dataset Search Results
published: 2025-10-01
Wang, Yajie; Huang, Xiaoqiang; Hui, Jingshu; Vo, Lam Tung; Zhao, Huimin (2025): Data from Stereoconvergent Reduction of Activated Alkenes by a Nicotinamide Free Synergistic Photobiocatalytic System. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-7433689_V1
There is a growing interest in developing cooperative chemoenzymatic reactions to harness the reactivity of chemical catalysts and the selectivity of enzymes for the synthesis of nonracemic chiral compounds. However, existing chemoenzymatic systems with more than one chemical reaction and one enzymatic reaction working cooperatively are rare. Moreover, the application of oxidoreductases in cooperative chemoenzymatic reactions is limited by the necessity of using expensive and unstable redox equivalents such as nicotinamide cofactors. Here, we report a light-driven cooperative chemoenzymatic system comprised of a photoinduced electron transfer reaction (PET) and a photosensitized energy transfer reaction (PEnT) with an enzymatic reduction in one-pot to synthesize chiral building blocks of bioactive compounds. As a proof of concept, ene-reductase was directly regenerated by PET in the absence of external cofactors. Meanwhile, enzymatic reduction worked cooperatively with photocatalyst-catalyzed energy transfer that continuously replenished the reactive isomer from the less reactive one. The whole system stereoconvergently reduced E/Z mixtures of alkenes to the enantiopure products. Additionally, enantioselective enzymatic reduction worked competitively with photocatalyst-catalyzed racemic background reaction and side reactions to channel the overall electron flow to the single enantiopure product. Such a light-driven cooperative chemoenzymatic system holds great potential for asymmetric synthesis using inexpensive petroleum or biomass-derived alkenes.
keywords:
Conversion;Catalysis
published: 2025-10-01
Dai, Tao; Ellebracht, Nathan; Hunter Sellars, Elwin; Aui, Alvina; Hanna, Goldstein; Li, Wenqin; Hellwinckel, Chad; Price, Lydia; Wong, Andrew; Nico, Peter; Basso, Bruno; Robertson, G Philip; Pett-Ridge, Jennifer; Langholtz, Matthew; Baker, Sarah; Pang, Simon; Scown, Corinne (2025): Data for "Land-based Resources for Engineered Carbon Dioxide Removal in the United States Exceed the Expected Needs". University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-2796463_V1
Gigatonne-scale atmospheric carbon dioxide removal (CDR), alongside deep emission cuts, is critical to stabilizing the climate. However, some of the most scalable CDR technologies are also the most land intensive. Here, we examine whether adequate land resources exist in the contiguous United States to meet CDR targets when prioritizing grid emissions reduction, food production, and the protection of sensitive ecosystems. We focus on biomass carbon removal and storage (BiCRS) and direct air capture and storage (DACS) and show that suitable lands exceed the expected needs: 37.6 million hectares of land are available for BiCRS, resulting in 0.26 GtCO2 of CDR/year, and 34 million hectares are suitable for wind- and solar-powered DACS, resulting in 4.8 GtCO2 of CDR/year if facilities are co-located with geologic CO2 storage. We identify biomass and energy supply hotspots to meet CDR targets while ensuring land protection and minimizing land competition.
keywords:
carbon; geospatial
published: 2025-10-01
Lyu, Mingkuan; Kong, Linggen; Yang, Zhenglin; Wu, Yuting; McGhee, Claire E.; Lu, Yi (2025): Data from PNA-Assisted DNAzymes to Cleave Double-Stranded DNA for Genetic Engineering with High Sequence Fidelity. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-1858504_V1
DNAzymes have been widely used in many sensing and imaging applications but have rarely been used for genetic engineering since their discovery in 1994, because their substrate scope is mostly limited to single-stranded DNA or RNA, whereas genetic information is stored mostly in double-stranded DNA (dsDNA). To overcome this major limitation, we herein report peptide nucleic acid (PNA)-assisted double-stranded DNA nicking by DNAzymes (PANDA) as the first example to expand DNAzyme activity toward dsDNA. We show that PANDA is programmable in efficiently nicking or causing double strand breaks on target dsDNA, which mimics protein nucleases and can act as restriction enzymes in molecular cloning. In addition to being much smaller than protein enzymes, PANDA has a higher sequence fidelity compared with CRISPR/Cas under the condition we tested, demonstrating its potential as a novel alternative tool for genetic engineering and other biochemical applications.
keywords:
Conversion;Genomics;Genome Engineering
published: 2025-10-01
Schetter, August; Lin, Cheng-Hsien; Zumpf, Colleen; Jang, Chunhwa; Hoffmann Jr., Leo; Rooney, William; Lee, DoKyoung (2025): Data from Genotype-Environment-Management Interactions in Biomass Yield and Feedstock Composition of Photoperiod-Sensitive Energy Sorghum. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-9321781_V1
Recently introduced photoperiod-sensitive (PS) biomass sorghum (Sorghum bicolor L. Moench) needs to be investigated for yield potential under different cultivation environments with reasonable nitrogen (N) inputs. The objectives of this study were to (1) evaluate the biomass yield and feedstock quality of four sorghum hybrids with different levels of PS ranging from very PS (VPS) hybrids and to moderate PS (MPS) hybrids, and (2) determine the optimal N inputs (0~168 kg N ha−1) under four environments: combinations of both temperate (Urbana, IL) and subtropical (College Station, TX) regions during 2018 and 2019. Compared to TX, the PS sorghums in central IL showed higher yield potential and steady feedstock production with an extended day length and with less precipitation variability, especially for the VPS hybrids. The mean dry matter (DM) yields of VPS hybrids were 20.5 Mg DM ha−1 and 17.7 Mg DM ha−1 in IL and TX, respectively. The highest N use efficiency occurred at a low N rate of 56 kg N ha−1 by improving approximately 33 kg DM ha−1 per 1.0 kg N ha−1 input. Approximately 70% of the PS sorghum biomass can be utilized for biofuel production, consisting of 58-65% of the cell-wall components and 4-11% of the soluble sugar. This study demonstrated that the rainfed temperate area (e.g., IL) has a great potential for the sustainable cultivation of PS energy sorghum due to their observed high yield potential, stable production, and low N requirements.
keywords:
Sustainability;Biomass Analytics;Field Data
published: 2025-08-13
Tang, Wenhan; Arabas, Sylwester; Curtis, Jeffrey H.; Knopf, Daniel A.; West, Matthew; Riemer, Nicole (2025): Data for: The impact of aerosol mixing state on immersion freezing: Insights from classical nucleation theory and particle-resolved simulations. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-6849781_V1
This dataset contains the values directly shown in the figures of the article "The impact of aerosol mixing state on immersion freezing: Insights from classical nucleation theory and particle-resolved simulations". This article is in preparation for submission to the journal Atmospheric Chemistry and Physics. The dataset consists of 15 NetCDF files processed from the raw output of the PartMC model. It does not include the theoretical values of frozen fraction, which can be computed using the equations provided in the paper.
keywords:
Aerosol mixing state; Ice nucleating particles; Classical nucleation theory
published: 2025-10-01
Crawford, Reed; Wolff, Patrick; Pierce, Ellen; Braun de Torrez, Elizabeth; Pourshoushtari, Roxanne; O'Keefe, Joy (2025): Dataset for Calling home: testing the efficacy of an acoustic lure and novel roost designs for attracting a rare bat. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-0959838_V1
This dataset contains the raw Florida bonneted bat echolocation calls recorded in southern Florida, USA from the years 2021 and 2022. This dataset also includes our artificial roost microclimate data (2021 only) and observations of bats recorded in our artificial roosts (2021 and 2022). Lastly, we include the R script required to analyze the Florida bonneted bat echolocation calls and the R script to produce the supplemental table and supplemental figure for our microclimate data.
keywords:
bats; roosts; acoustics
published: 2023-05-02
Lee, Jou; Schneider, Jodi (2023): Crossref data for Assessing the agreement in retraction indexing across 4 multidisciplinary sources: Crossref, Retraction Watch, Scopus, and Web of Science. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-9099305_V1
Tab-separated value (TSV) file. 14745 data rows. Each data row represents publication metadata as retrieved from Crossref (http://crossref.org) 2023-04-05 when searching for retracted publications. Each row has the following columns: Index - Our index, starting with 0. DOI - Digital Object Identifier (DOI) for the publication Year - Publication year associated with the DOI. URL - Web location associated with the DOI. Title - Title associated with the DOI. May be blank. Author - Author(s) associated with the DOI. Journal - Publication venue (journal, conference, ...) associated with the DOI RetractionYear - Retraction Year associated with the DOI. May be blank. Category - One or more categories associated with the DOI. May be blank. Our search was via the Crossref REST API and searched for: Update_type=( 'retraction', 'Retraction', 'retracion', 'retration', 'partial_retraction', 'withdrawal','removal')
keywords:
retraction; metadata; Crossref; RISRS
published: 2025-08-27
Jang, Chunhwa; Namoi, Nictor; Lee, Jung Woo; Becker, Talon; Rooney, William; Lee, DoKyoung (2025): Data for Identifying the best high-biomass sorghum hybrids based on biomass yield potential and feedstock quality affected by nitrogen fertility management under various environments. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-0618787_V1
Data were collected from agronomy fields in Urbana and Ewing, IL, during the 2022 and 2023 growing seasons. The dataset includes dry biomass yield, nitrogen, phosphorus, and potassium concentrations and removals, and chemical composition elements (cellulose, hemicellulose, lignin, and soluble fractions) for 13 high-biomass sorghum hybrids. data_sharing.xlsx contains 20 columns and 104 rows. Below is the explanation of all variables in the file: Year: 2022; 2023 Location: Urbana, IL; Ewing, IL N rate (kg-N/ha): 0; 112 Hybrid #: H1-H13 Pedigree: Pedigree for 13 hybrids Dry biomass yield (Mg/ha): Aboveground dry biomass yield N (g/kg): Nitrogen concentration in plant tissue P (g/kg): Phosphorus concentration in plant tissue K (g/kg): Potassium concentration in plant tissue N (kg/ha): Nitrogen removal by aboveground biomass P (kg/ha): Phosphorus removal by aboveground biomass K (kg/ha): Potassium removal by aboveground biomass Cellulose (g/kg): Cellulose concentration in plant tissue Hemicellulose (g/kg): Hemicellulose concentration in plant tissue Lignin (g/kg): Lignin concentration in plant tissue Soluble (g/kg): Soluble concentration in plant tissue Cellulose (Mg/ha): Cellulose content in aboveground biomass Hemicellulose (Mg/ha): Hemicellulose content in aboveground biomass Lignin (Mg/ha): Lignin content in aboveground biomass Soluble (Mg/ha): Soluble content in aboveground biomass
keywords:
high-biomass sorghum hybrids; yield potential; environmental adaptability; feedstock quality; nutrient removal; N fertilization
published: 2025-09-30
Huber, George; Guest, Jeremy; Santiago-Martinez, Leoncio; Bhagwat, Sarang; Kim, Min Soo (2025): Data for "Sustainable Potassium Sorbate Production from Triacetic Acid Lactone in Food-Grade Solvents". University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-0247002_V1
This study advances the production of potassium sorbate (KS) from triacetic acid lactone (TAL) utilizing food-grade solvents, ethanol (EtOH) and isopropyl alcohol (IPA). We have previously demonstrated the route to produce KS from TAL in tetrahydrofuran (THF) as the main solvent, but the use of THF is associated with environmental and health risks especially for food applications. The process employs a catalytic approach in food-grade solvents and includes three main steps: hydrogenation, etherification and hydrolysis, and ring-opening hydrolysis to produce KS from TAL. In the synthesis of KS from TAL, the use of IPA leads to higher yields and reduced reaction times compared to EtOH. As a result, the overall reaction time in IPA was reduced to 35.7 h, compared to 42.1 h in our previous study using THF and EtOH, while achieving a comparable KS yield of 84% from TAL. The synthesized KS exhibits a trans-2, trans-4 geometrical configuration, identical to that of commercially available KS. Through techno-economic analysis (TEA) and life cycle assessment (LCA), we estimated full-scale production of KS from sugarcane with the developed process in IPA could achieve a minimum product selling price (MPSP) of $8.27 per kg with a range of $7.06–10.16 per kg [5th–95th percentiles from 6000 Monte Carlo simulations] and a carbon intensity (CI) of 13.7 [9.6–18.6] kg CO2-eq per kg. This study highlights the synthesis of KS from TAL using food-grade solvents, demonstrating improved economic viability and environmental sustainability compared to our previous research (MPSP of $9.68 per kg [$8.47–11.45 per kg] and CI of 16.2 [12.0–21.2] kg CO2-eq per kg), as the total required reaction decreases while achieving the comparable overall yield of KS from TAL.
keywords:
bioproducts; catalysis
published: 2025-09-30
Kurambhatti, Chinmay V.; Kumar, Deepak; Singh, Vijay (2025): Data from Technical and Economic Feasibility of an Integrated Ethanol and Anthocyanin Coproduction Process Using Purple Corn Stover. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-3508511_V1
The coproduction of high-value anthocyanin extract in the cellulosic ethanol process would diversify the co-product market, increase revenue, and potentially improve the economics of the process. The high anthocyanin concentration in the cob and structural carbohydrates in residual stover make purple corn stover an attractive source for anthocyanin and ethanol coproduction. This study aimed to develop simulation models for processes integrating ethanol production and anthocyanin extraction using purple corn stover, to evaluate their techno-economic feasibility, and to compare their performance with the conventional ethanol production process using corn stover. The annual ethanol production for plants processing 2000 MT dry feedstock / day was 148.6 million L/year for the integrated processes compared with 222.6 million L/year for the conventional process. Anthocyanin production in the modified processes using dilute acid-based and water-based anthocyanin extraction processes was 1779 and 1099 MT/year, respectively. Capital investments for the integrated processes ($448.1 to $443.8 million) were higher than the conventional process ($371.9 million). Due to high revenue from anthocyanin extract, the ethanol production cost for the integrated process using acid-based anthocyanin extraction ($0.36/L) was 34.5% lower than conventional ethanol production ($0.55/L). The ethanol production cost for the integrated process using water-based anthocyanin extraction ($0.68/L) was higher than conventional ethanol production due to low ethanol and anthocyanin yields. The minimum ethanol selling price for the integrated process using acid-based anthocyanin extraction ($0.65/L) was also lower than the conventional process ($0.72/L), indicating an improvement in economic performance.
keywords:
Conversion;Economics;Feedstock Bioprocessing;Modeling
published: 2025-09-30
Viswanathan, Mothi Bharath; Cheng, Ming-Hsun; Clemente, Tom; Dweikat, Ismail; Singh, Vijay (2025): Data from Economic Perspective of Ethanol and Biodiesel Coproduction from Industrial Hemp. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-1141487_V1
In this study, the economics of producing biofuels from an industrial hemp (Cannabis sativa) genotype – 19m96136 was investigated. A lignocellulosic biofuel plant, hourly consuming 85 metric tons of hemp biomass was modeled in SuperPro Designer®. The integrated bioenergy plant produced hemp biodiesel and bioethanol from lipids and carbohydrates, respectively. The structural composition of the industrial hemp plant was analyzed in a previous study. The data obtained was used to simulate feedstock composition in SuperPro Designer®. The simulation results indicated that Hemp containing 2% lipids can yield up to 3.95 million gallons of biodiesel annually. On improving biomass lipid content to 5 and 10%, biodiesel production increased to 9.88 and 19.91 million gallons, respectively. The breakeven unit production cost of hemp biodiesel with 2, 5, and 10% lipid containing hemp was $18.49, $7.87, and $4.13/gallon, respectively. The biodiesel unit production cost when utilizing 10% lipid-containing hemp was comparable to soybean biodiesel at $4.13/gallon. Furthermore, sensitivity analysis revealed the possibility of a 7.80% reduction in unit production cost upon a 10% reduction in hemp feedstock cost. Furthermore, industrial hemp was capable of producing between 307.80 and 325.82 gallons of total biofuels per hectare of agricultural land than soybean.
keywords:
Conversion;Feedstock Production;Economics;Modeling
published: 2025-09-30
Yun, Danim; Ayla, E. Zeynep; Bregante, Daniel T.; Flaherty, David W. (2025): Data from Reactive Species and Reaction Pathways for the Oxidative Cleavage of 4-Octene and Oleic Acid with H2O2 over Tungsten Oxide Catalysts. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-6782711_V1
Oxidative cleavage of carbon–carbon double bonds (C═C) in alkenes and fatty acids produces aldehydes and acids valued as chemical intermediates. Solid tungsten oxide catalysts are low cost, nontoxic, and selective for the oxidative cleavage of C═C bonds with hydrogen peroxide (H2O2) and are, therefore, a promising option for continuous processes. Despite the relevance of these materials, the elementary steps involved and their sensitivity to the form of W sites present on surfaces have not been described. Here, we combine in situ spectroscopy and rate measurements to identify significant steps in the reaction and the reactive species present on the catalysts and examine differences between the kinetics of this reaction on isolated W atoms grafted to alumina and on those exposed on crystalline WO3 nanoparticles. Raman spectroscopy shows that W–peroxo complexes (W–(η2-O2)) formed from H2O2 react with alkenes in a kinetically relevant step to produce epoxides, which undergo hydrolysis at protic surface sites. Subsequently, the CH3CN solvent deprotonates diols to form alpha-hydroxy ketones that react to form aldehydes and water following nucleophilic attack of H2O2. Turnover rates for oxidative cleavage, determined by in situ site titrations, on WOx–Al2O3 are 75% greater than those on WO3 at standard conditions. These differences reflect the activation enthalpies (ΔH‡) for the oxidative cleavage of 4-octene that are much lower than those for the isolated WOx sites (36 ± 3 and 60 ± 6 kJ·mol–1 for WOx–Al2O3 and WO3, respectively) and correlate strongly with the difference between the enthalpies of adsorption for epoxyoctane (ΔHads,epox), which resembles the transition state for epoxidation. The WOx–Al2O3 catalysts mediate oxidative cleavage of oleic acid with H2O2 following a mechanism comparable to that for the oxidative cleavage of 4-octene. The WO3 materials, however, form only the epoxide and do not cleave the C–C bond or produce aldehydes and acids. These differences reflect the distinct site requirements for these reaction pathways and indicate that acid sites required for diol formation are strongly inhibited by oleic acids and epoxides on WO3 whereas the Al2O3 support provides sites competent for this reaction and increase the yield of the oxidative cleavage products.
keywords:
Catalysis;Conversion
published: 2025-09-29
Wang, Sheng; Guan, Kaiyu; Wang, Zhihui; Ainsworth, Elizabeth; Zheng, Ting; Townsend, Philip; Li, Kaiyuan; Moller, Christopher; Wu, Genghong; Jiang, Chongya (2025): Data from Unique Contributions of Chlorophyll and Nitrogen to Predict Crop Photosynthetic Capacity from Leaf Spectroscopy. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-2124827_V1
The photosynthetic capacity or the CO2-saturated photosynthetic rate (Vmax), chlorophyll, and nitrogen are closely linked leaf traits that determine C4 crop photosynthesis and yield. Accurate, timely, rapid, and non-destructive approaches to predict leaf photosynthetic traits from hyperspectral reflectance are urgently needed for high-throughput crop monitoring to ensure food and bioenergy security. Therefore, this study thoroughly evaluated the state-of-the-art physically based radiative transfer models (RTMs), data-driven partial least squares regression (PLSR), and generalized PLSR (gPLSR) models to estimate leaf traits from leaf-clip hyperspectral reflectance, which was collected from maize (Zea mays L.) bioenergy plots with diverse genotypes, growth stages, treatments with nitrogen fertilizers, and ozone stresses in three growing seasons. The results show that leaf RTMs considering bidirectional effects can give accurate estimates of chlorophyll content (Pearson correlation r=0.95), while gPLSR enabled retrieval of leaf nitrogen concentration (r=0.85). Using PLSR with field measurements for training, the cross-validation indicates that Vmax can be well predicted from spectra (r=0.81). The integration of chlorophyll content (strongly related to visible spectra) and nitrogen concentration (linked to shortwave infrared signals) can provide better predictions of Vmax (r=0.71) than only using either chlorophyll or nitrogen individually. This study highlights that leaf chlorophyll content and nitrogen concentration have key and unique contributions to Vmax prediction.
keywords:
Feedstock Production;Sustainability;Biomass Analytics;Modeling
published: 2025-09-29
Frederick, Samuel; Mohebalhojeh, Matin; Curtis, Jeffrey; West, Matthew; Riemer, Nicole (2025): Data for "Idealized Particle-Resolved Large-Eddy Simulations to Evaluate the Impact of Emissions Spatial Heterogeneity on CCN Activity". University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-9622921_V2
This dateset contains data files necessary to replicate figures from "Idealized Particle-Resolved Large-Eddy Simulations to Evaluate the Impact of Emissions Spatial Heterogeneity on CCN Activity" submitted to Atmospheric Chemistry and Physics. Within the compressed folder data.zip are two subdirectories, "processed_data" and "spatial-het". The "processed_data" directory contains netCDF files which contain a subset of simulation output used in figure generation. The "spatial-het" subdirectory contains a .csv file with spatial heterogeneity values computed via an exact algorithm of the spatial heterogeneity metric described by Mohebalhojeh et al. 2025. The subdirectory "sh-patterns" contains .csv files for each emissions scenario. Each entry corresponds to a single grid cell over a domain of dimension 100x100 (lateral resolution of the computational domain employed in this paper). Within scripts.zip are python notebooks for generating figures. Additional python modules are included which contain helper functions for notebooks. Furthermore, a Fortran version of the spatial heterogeneity metric is included alongside shells scripts for creating a python environment in which the code can be compiled and convert into a Python module. Note that the create_env.sh and compile_nsh.sh scripts must be run prior to executing cells in notebooks to make use of the spatial heterogeneity subroutines. <b>*Note*:</b> New in this V2: Following an initial review, an additional figure was requested, which required updates to both data.zip (adding one new NC file: no-heterogeneity_met-vars_subset.nc) and scripts.zip (a minor addition to a Python notebook). A README in PDF format has also been uploaded to provide a summary of the dataset.
keywords:
Atmospheric chemistry; aerosols; Particle-resolved modeling; spatial heterogeneity
published: 2025-09-29
Zhai, Zhiyang; Liu, Hui; Shanklin, John (2025): OWD.2 TAG Expression. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-0016423_V1
During the transformation of wild-type (WT) Arabidopsis thaliana, a T-DNA containing OLEOSIN-GFP (OLE1-GFP) was inserted by happenstance within the GBSS1 gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining gbss1 with the expression of OLE1-GFP was confirmed by transforming an independent gbss1 mutant (GABI_914G01) with OLE1-GFP. The resulting OLE1-GFP/gbss1 transgenic lines showed higher leaf oil content than the individual OLE1-GFP/WT or single gbss1 mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in OG significantly elevated its oil content in mature leaves to 2.3% of dry weight, which is 15 times higher than that in WT Arabidopsis. Inducible expression of the same lipogenic factors was shown to be an effective strategy for triacylglycerol (TAG) accumulation without incurring growth, development, and yield penalties.
keywords:
Feedstock Production;Biomass Analytics
published: 2025-09-29
Li, Shuai; Moller, Christopher; Mitchell, Noah G.; Lee, DoKyoung; Ainsworth, Elizabeth (2025): Data from Bioenergy Sorghum Maintains Photosynthetic Capacity in Elevated Ozone Concentrations. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-8087553_V1
Elevated tropospheric ozone concentration (O3) significantly reduces photosynthesis and productivity in several C4 crops including maize, switchgrass and sugarcane. However, it is unknown how O3 affects plant growth, development and productivity in sorghum (Sorghum bicolor L.), an emerging C4 bioenergy crop. Here, we investigated the effects of elevated O3 on photosynthesis, biomass and nutrient composition of a number of sorghum genotypes over two seasons in the field using free-air concentration enrichment (FACE), and in growth chambers. We also tested if elevated O3 altered the relationship between stomatal conductance and environmental conditions using two common stomatal conductance models. Sorghum genotypes showed significant variability in plant functional traits, including photosynthetic capacity, leaf N content and specific leaf area, but responded similarly to O3. At the FACE experiment, elevated O3 did not alter net CO2 assimilation (A), stomatal conductance (gs), stomatal sensitivity to the environment, chlorophyll fluorescence and plant biomass, but led to reductions in the maximum carboxylation capacity of phosphoenolpyruvate and increased stomatal limitation to A in both years. These findings suggest that bioenergy sorghum is tolerant to O3 and could be used to enhance biomass productivity in O3 polluted regions.
keywords:
Feedstock Production;Sustainability;Field Data
published: 2025-09-29
Singh, Vijay; Kurambhatti, Chinmay V.; Kumar, Deepak; Rausch, Kent; Tumbleson, M.E. (2025): Data from Ethanol Production from Corn Fiber Separated after Liquefaction in the Dry Grind Process. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-8118582_V1
Conversion of corn fiber to ethanol in the dry grind process can increase ethanol yields, improve coproduct quality and contribute to process sustainability. This work investigates the use of two physio-chemical pretreatments on corn fiber and effect of cellulase enzyme dosage to improve ethanol yields. Fiber separated after liquefaction of corn was pretreated using (1) hot water pretreatment (160°C for 5, 10 or 20 min); and (2) wet disk milling and converted to ethanol. The conversion efficiencies of hot water pretreated fiber were higher than untreated fiber, with highest increase in conversion (10.4%) achieved for 5-minute residence time at 160 °C. Disk milling was not effective in increasing conversion compared to other treatments. Hydrolysis and fermentation of untreated fiber with excess cellulase enzymes resulted in 33.3% higher conversion compared to untreated fiber. Note: in “Table1_Treatments.csv”, NA = Not applicable.
keywords:
Conversion;Feedstock Bioprocessing
published: 2025-09-29
Guo, Zhihui; Xu, Meilan; Nagano, Hironori; Clark, Lindsay; Sacks, Erik; Yamada, Toshihiko (2025): Data for Characterization of the Ghd8 Flowering Time Gene in a Mini-Core Collection of Miscanthus sinensis. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-4196462_V1
The optimal flowering time for bioenergy crop miscanthus is essential for environmental adaptability and biomass accumulation. However, little is known about how genes controlling flowering in other grasses contribute to flowering regulation in miscanthus. Here, we report on the sequence characterization and gene expression of Miscanthus sinensisGhd8, a transcription factor encoding a HAP3/NF-YB DNA-binding domain, which has been identified as a major quantitative trait locus in rice, with pleiotropic effects on grain yield, heading date and plant height. In M. sinensis, we identified two homoeologous loci, MsiGhd8A located on chromosome 13 and MsiGhd8B on chromosome 7, with one on each of this paleo-allotetraploid species’ subgenomes. A total of 46 alleles and 28 predicted protein sequence types were identified in 12 wild-collected accessions. Several variants of MsiGhd8 showed a geographic and latitudinal distribution. Quantitative real-time PCR revealed that MsiGhd8 expressed under both long days and short days, and MsiGhd8B showed a significantly higher expression than MsiGhd8A. The comparison between flowering time and gene expression indicated that MsiGhd8B affected flowering time in response to day length for some accessions. This study provides insight into the conserved function of Ghd8 in the Poaceae, and is an important initial step in elucidating the flowering regulatory network of Miscanthus.
keywords:
Feedstock Production;Genomics
published: 2025-09-29
Blanc-Betes, Elena (2025): DayCent MUVP model calibrated for the simulation of bioenergy crops grown on set-aside land in the US. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-7710407_V1
DayCent MUVP version (Methanogenesis, UV litter degradation and Photosynthesis). DAYCENT is the daily time-step version of the CENTURY biogeochemical model (Parton et al., 1994). DAYCENT simulates fluxes of C and N among the atmosphere, vegetation, and soil (Del Grosso et al., 2001a; Parton et al., 1998). Key submodels include soil water content and temperature by layer, plant production and allocation of net primary production (NPP), decomposition of litter and soil organic matter, mineralization of nutrients, N gas emissions from nitrification and denitrification, and CH4 oxidation in non-saturated soils.
keywords:
biogeochemical model
published: 2025-09-26
Arora, Amit; Singh, Vijay (2025): Data from Biodiesel Production from Engineered Sugarcane Lipids under Uncertain Feedstock Compositions: Process Design and Techno-Economic Analysis. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-6179868_V1
In this study, different process schemes were designed and evaluated for biodiesel production from engineered cane lipids with uncertain fatty acid compositions. Four different process schemes were compared under (i) thermal glycerolysis and (ii) enzymatic glycerolysis approaches. These schemes were based on the biodiesel yield and economic indicators such as the net present value (NPV) and the minimum selling price (MSP) of biodiesel. A scheme with polar lipid separation under thermal glycerolysis resulted in the maximum NPV ($96.5 million) and minimum MSP ($1107/ton biodiesel), respectively. Through local sensitivity analysis, it was concluded that the cane lipid percentage is the most significant factor influencing process economics. A conjoint analysis of the lipid procurement price and cane lipid percent suggested that 15% cane lipids with a low lipid procurement price ($0.536/kg) results in a positive NPV. When the cane lipid price is higher (>$0.80/kg), a 20% lipid content should be considered to achieve a positive NPV. At 20% cane lipids, the worst-case and best-case scenarios were evaluated by analyzing the interplay of the three most important parameters, The best-case scenario revealed that the minimum NPV under any process scheme could yield more than $100 million (or MSP: $0.80/L), and the worst-case analysis showed that losses incurred by the plant could be as high as $80 million (MSP: $1.36/L). A Monte Carlo simulation indicated that there is a 70% chance of the plant being profitable (NPV > 0).
keywords:
Conversion;Economics;Feedstock Bioprocessing;Modeling
published: 2025-09-25
Huang, Yijing; Abboud, Nick (2025): Data for Observation of a Dynamic Magneto-chiral Instability in Photoexcited Tellurium. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-1409842_V3
This repository provides the data and code used to reproduce key plots from the manuscript and to extend discussions that were only briefly covered therein. All MATLAB scripts were developed and tested in MATLAB R2024a. All Python scripts were developed and tested in Python 3.11.2. * <b> NOTE:</b> New in this V3: 1. 2 new MATLAB files (ChiralPointGroups.m and THz_current_estimation.m), ChiralPointGroups.pdf (a compiled version of ChiralPointGroups.m) and theoretical model code (theoretical_model.zip) are added. More information can be found in the readme. 2. Updated and renamed "publication_data.zip" (in V2) to "data_and_analysis.zip" 3. Change License from CC BY to "Other license". Licensing Terms: Data (all .mat files) is under CC BY and Code is released under MIT license. Therefore, V3 is bound to this new license. V2 is still under CC BY. <b>→ Data and analysis code (data_and_analysis.zip):</b> The dataset is organized into five subfolders. Each subfolder corresponds to a unique combination of experimental conditions, including: • Magnetic field orientation (B ∥ c or B ⟂ c) • Scan parameter (magnetic field or temperature) • Pump laser polarization (linear s, linear p, or circular) • Detection polarization (linear s) Each folder contains: • The raw time-domain data files (.mat) • Oscillator parameters extracted via linear prediction algorithm (.mat) • MATLAB scripts (.m) that generate plots of the raw data, processed fits, and amplified modes. Each script should be run within its corresponding folder to ensure proper loading of the associated data files. Folder summary: 1. B_parallel_c_linear_spump_sprobe_field: B ∥ c, s-polarized pump, s-polarized THz detection, magnetic field dependence 2. B_parallel_c_linear_spump_sprobe_temperature: B ∥ c, s-polarized pump, s-polarized THz detection, temperature dependence 3. B_perp_c_linear_spump_sprobe_field: B ⟂ c, s-polarized pump, s-polarized THz detection, magnetic field dependence 4. B_perp_c_linear_spump_sprobe_temperature: B ⟂ c, s-polarized pump, s-polarized THz detection, temperature dependence 5. B_parallel_c_LCPRCP_pump_sprobe_field: B ∥ c, circularly polarized pump (LCP & RCP), s-polarized THz detection, magnetic field dependence <b>→Theoretical model code (theoretical_model.zip):</b> The Python script depends on packages “numpy” and “matplotlib”. The script generates a plot of the dispersion relations of the theoretical model introduced in the Main Text. More precisely, it plots the real (red) and imaginary (blue) parts of the frequency (ω) as a function of wavenumber (k) as obtained by solving the characteristic equation, equation (6) of the Supplemental Information, with σ_E and σ_Μ given respectively by equations (3) and (2) of the Main Text. All branches of the dispersion relations are plotted simultaneously. All model parameters are adjustable. The included Mathematica notebook (printout also provided in .pdf format) was used to obtain symbolic expressions for the coefficients of powers of ω appearing in the characteristic determinant. These coefficients were copied directly into the Python function detCoeffs(). <b>→ Standalone scripts (not in subfolders):</b> • ChiralPointGroups.m Outputs a table summarizing the 2D matrix representation of σ_Μ in the 11 enantiomorphic point groups. ChiralPointGroups.pdf is a compiled version of chiral point groups table, identical to the output of ChiralPointGroups.m. • THz_current_estimation.m Estimates the photoinduced THz current in tellurium under magnetic field. The script evaluates a phenomenological resonant contribution to the magnetoelectric coupling (with negligible dependence on NIR polarization), leading to excitation of s-polarized, B-antisymmetric mode S_odd at ~0.37 THz. These standalone scripts provide additional physical discussion and calculation detail that are intentionally streamlined or omitted from the published manuscript and its supplementary materials for clarity and space.
keywords:
magneto-chiral instability; THz emission; THz spectroscopy; nonequilibrium states; emergent phenomena; Weyl semiconductor; tellurium; ultrafast spectrscopy; photoexcitation
published: 2025-09-26
Dong, Hongxu; Clark, Lindsay; Jin, Xiaoli; Anzoua, Kossonou; Bagmet, Larisa; Chebukin, Pavel; Dzyubenko, Elena; Dzyubenko, Nicolay; Ghimire, Bimal Kumar; Heo, Kweon; Johnson, Douglas A.; Nagano, Hironori; Sabitov, Andrey; Peng, Junhua; Yamada, Toshihiko; Yoo, Ji Hye; Yu, Chang Yeon; Zhao, Hua; Long, Stephen P.; Sacks, Erik (2025): Data from Managing Flowering Time in Miscanthus and Sugarcane to Facilitate Intra- and Intergeneric Crosses. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-1470836_V1
Miscanthus is a close relative of saccharum and a potentially valuable genetic resource for improving sugarcane. Differences in flowering time within and between miscanthus and saccharum hinders intra- and interspecific hybridizations. A series of greenhouse experiments were conducted over three years to determine how to synchronize flowering time of saccharum and miscanthus genotypes. We found that day length was an important factor influencing when miscanthus and saccharum flowered. Sugarcane could be induced to flower in a central Illinois greenhouse using supplemental lighting to reduce the rate at which days shortened during the autumn and winter to 1 min d-1, which allowed us to synchronize the flowering of some sugarcane genotypes with Miscanthus genotypes primarily from low latitudes. In a complementary growth chamber experiment, we evaluated 33 miscanthus genotypes, including 28 M. sinensis, 2 M. floridulus, and 3 M. ×giganteus collected from 20.9° S to 44.9° N for response to three day lengths (10 h, 12.5 h, and 15 h). High latitude-adapted M. sinensis flowered mainly under 15 h days, but unexpectedly, short days resulted in short, stocky plants that did not flower; in some cases, flag leaves developed under short days but heading did not occur. In contrast, for M. sinensis and M. floridulus from low latitudes, shorter day lengths typically resulted in earlier flowering, and for some low latitude genotypes, 15 h days resulted in no flowering. However, the highest ratio of reproductive shoots to total number of culms was typically observed for 12.5 h or 15 h days. Latitude of origin was significantly associated with culm length, and the shorter the days, the stronger the relationship. Nearly all entries achieved maximal culm length under the 15 h treatment, but the nearer to the equator an accession originated, the less of a difference in culm length between the short-day treatments and the 15 h day treatment. Under short days, short culms for high-latitude accessions was achieved by different physiological mechanisms for M. sinensis genetic groups from the mainland in comparison to those from Japan; for mainland accessions, the mechanism was reduced internode length, whereas for Japanese accessions the phyllochron under short days was greater than under long days. Thus, for M. sinensis, short days typically hastened floral induction, consistent with the expectations for a facultative short-day plant. However, for high latitude accessions of M. sinensis, days less than 12.5 h also signaled that plants should prepare for winter by producing many short culms with limited elongation and development; moreover, this response was also epistatic to flowering. Thus, to flower M. sinensis that originates from high latitudes synchronously with sugarcane, the former needs day lengths >12.5 h (perhaps as high as 15 h), whereas that the latter needs day lengths <12.5 h.
keywords:
Feedstock Production;Phenomics
published: 2025-09-25
Vu-Le, The-Anh; Park, Minhyuk; Chen, Ian; Warnow, Tandy (2025): Data for "Using Stochastic Block Models for Community Detection". University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-3421614_V1
Dataset for "Using Stochastic Block Models for Community Detection". This contains synthetic networks with ground-truth community structure generated using synthetic network generators (specifically, ABCD+o) based on real-world networks and computed clusterings on these real-world networks. Note: * networks.zip contains the synthetic networks
published: 2025-09-25
Moore, Caitlin E.; von Haden, Adam C.; Burnham, Mark B.; Kantola, Ilsa B.; Gibson, Christy; Blakely, Bethany; Dracup, Evan; Masters, Michael D.; Yang, Wendy; DeLucia, Evan H.; Bernacchi, Carl (2025): Biogeochemical Fluxes for Three Bioenergy Crops. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-8157843_V1
Perennial crops have been the focus of bioenergy research and development for their sustainability benefits associated with high soil carbon (C) and reduced nitrogen (N) requirements. However, perennial crops mature over several years and their sustainability benefits can be negated through land reversion. A photoperiod‐sensitive energy sorghum (Sorghum bicolor) may provide an annual crop alternative more ecologically sustainable than maize (Zea mays) that can more easily integrate into crop rotations than perennials, such as miscanthus (Miscanthus × giganteus). This study presents an ecosystem‐scale comparison of C, N, water and energy fluxes from energy sorghum, maize and miscanthus during a typical growing season in the Midwest United States. Gross primary productivity (GPP) was highest for maize during the peak growing season at 21.83 g C m−2 day−1, followed by energy sorghum (17.04 g C m−2 day−1) and miscanthus (15.57 g C m−2 day−1). Maize also had the highest peak growing season evapotranspiration at 5.39 mm day−1, with energy sorghum and miscanthus at 3.81 and 3.61 mm day−1, respectively. Energy sorghum was the most efficient water user (WUE), while maize and miscanthus were comparatively similar (3.04, 1.75 and 1.89 g C mm−1 H2O, respectively). Maize albedo was lower than energy sorghum and miscanthus (0.19, 0.26 and 0.24, respectively), but energy sorghum had a Bowen ratio closer to maize than miscanthus (0.12, 0.13 and 0.21, respectively). Nitrous oxide (N2O) flux was higher from maize and energy sorghum (8.86 and 12.04 kg N ha−1, respectively) compared with miscanthus (0.51 kg N ha−1), indicative of their different agronomic management. These results are an important first look at how energy sorghum compares to maize and miscanthus grown in the Midwest United States. This quantitative assessment is a critical component for calibrating biogeochemical and ecological models used to forecast bioenergy crop growth, productivity and sustainability.
keywords:
Sustainability;Field Data
published: 2025-09-24
Lee, Jaewon; Kwak, Suryang; Liu, Jing-Jing; Yu, Sora; Yun, Eun Ju; Kim, Dong Hyun; Liu, Cassie; Kim, Kyoung Heon; Jin, Yong-Su (2025): Data from Enhanced 2′-Fucosyllactose Production by Engineered Saccharomyces cerevisiae using Xylose as a Co-Substrate. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-5204797_V1
2′-Fucosyllactose (2′-FL), a human milk oligosaccharide with confirmed benefits for infant health, is a promising infant formula ingredient. Although Escherichia coli, Saccharomyces cerevisiae, Corynebacterium glutamicum, and Bacillus subtilis have been engineered to produce 2′-FL, their titers and productivities need be improved for economic production. Glucose along with lactose have been used as substrates for producing 2′-FL, but accumulation of by-products due to overflow metabolism of glucose hampered efficient production of 2′-FL regardless of a host strain. To circumvent this problem, we used xylose, which is the second most abundant sugar in plant cell wall hydrolysates and is metabolized through oxidative metabolism, for the production of 2′-FL by engineered yeast. Specifically, we modified an engineered S. cerevisiae strain capable of assimilating xylose to produce 2′-FL from a mixture of xylose and lactose. First, a lactose transporter (Lac12) from Kluyveromyces lactis was introduced. Second, a heterologous 2′-FL biosynthetic pathway consisting of enzymes Gmd, WcaG, and WbgL from E. coli was introduced. Third, we adjusted expression levels of the heterologous genes to maximize 2′-FL production. The resulting engineered yeast produced 25.5 g/L of 2′-FL with a volumetric productivity of 0.35 g/L∙h in a fed-batch fermentation with lactose and xylose feeding to mitigate the glucose repression. Interestingly, the major location of produced 2′-FL by the engineered yeast can be changed using different culture media. While 72% of the produced 2′-FL was secreted when a complex medium was used, 82% of the produced 2′-FL remained inside the cells when a minimal medium was used. As yeast extract is already used as food and animal feed ingredients, 2′-FL enriched yeast extract can be produced cost-effectively using the 2′-FL-accumulating yeast cells.
keywords:
Conversion;Genome Engineering
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