Data for Purple Acid Phosphatase2 Stimulates a Futile Cycle of Lipid Synthesis and Degradation, and Mitigates the Negative Growth Effects of Triacylglycerol Accumulation in Vegetative Tissues

Cai, Yingqi; Zhai, Zhiyang; Blanford, Jantana; Liu, Hui; Shi, Hai; Schwender, Jorg; Xu, Changcheng; Shanklin, John

doi:10.13012/B2IDB-0843926_V1

Illinois Data Bank - Dataset

Data for Purple Acid Phosphatase2 Stimulates a Futile Cycle of Lipid Synthesis and Degradation, and Mitigates the Negative Growth Effects of Triacylglycerol Accumulation in Vegetative Tissues

Cite this dataset:

Cai, Yingqi; Zhai, Zhiyang; Blanford, Jantana; Liu, Hui; Shi, Hai; Schwender, Jorg; Xu, Changcheng; Shanklin, John (2025): Data for Purple Acid Phosphatase2 Stimulates a Futile Cycle of Lipid Synthesis and Degradation, and Mitigates the Negative Growth Effects of Triacylglycerol Accumulation in Vegetative Tissues. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-0843926_V1

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Dataset Description	Storage lipids (mostly triacylglycerols, TAGs) serve as an important energy and carbon reserve in plants, and hyperaccumulation of TAG in vegetative tissues can have negative effects on plant growth. Purple acid phosphatase2 (PAP2) was previously shown to affect carbon metabolism and boost plant growth. However, the effects of PAP2 on lipid metabolism remain unknown. Here, we demonstrated that PAP2 can stimulate a futile cycle of fatty acid (FA) synthesis and degradation, and mitigate negative growth effects associated with high accumulation of TAG in vegetative tissues. Constitutive expression of PAP2 in Arabidopsis thaliana enhanced both lipid synthesis and degradation in leaves and led to a substantial increase in seed oil yield. Suppressing lipid degradation in a PAP2-overexpressing line by disrupting sugar-dependent1 (SDP1), a predominant TAG lipase, significantly elevated vegetative TAG content and improved plant growth. Diverting FAs from membrane lipids to TAGs in PAP2-overexpressing plants by constitutively expressing phospholipid:diacylglycerol acyltransferase1 (PDAT1) greatly increased TAG content in vegetative tissues without compromising biomass yield. These results highlight the potential of combining PAP2 with TAG-promoting factors to enhance carbon assimilation, FA synthesis and allocation to TAGs for optimized plant growth and storage lipid accumulation in vegetative tissues.
Subject	Life Sciences
Keywords	Feedstock Production;Biomass Analytics;Lipidomics
License	CC BY
Funder	U.S. Department of Energy (DOE)-Grant:DE-SC0018420
Corresponding Creator	John Shanklin
Downloaded	577 times
Related Materials (1) Article Cai, Y., Zhai, Z., Blanford, J., Liu, H., Shi, H., Schwender, J., Xu, C., Shanklin, J. July 18, 2022. “Purple Acid Phosphatase2 Stimulates a Futile Cycle of Lipid Synthesis and Degradation and Mitigates the Negative Growth Effects of Triacylglycerol Accumulation in Vegetative Tissues.” New Phytologist. DOI: 10.1111/nph.18392.

Versions

Version	DOI	Comment	Publication Date
1	10.13012/B2IDB-0843926_V1		2025-10-17

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README.txt 21.9 KB File
Fig1a_C14Lipids.csv 1.63 KB File
Fig1b_SeedOilDryWeight.csv 367 Bytes File
Fig1c_SeedYieldPerPlant.csv 222 Bytes File
Fig1d_SeedOilPerPlant.csv 323 Bytes File
Fig2a_C14Lipids.csv 795 Bytes File
Fig2b_LeafTAG.csv 326 Bytes File
Fig2c_LeafTotalLipids.csv 375 Bytes File
Fig2d_LeafLipidDegradation.csv 386 Bytes File
Fig3b_ShootBiomass.csv 331 Bytes File
Fig3c_TAGContent2Weeks.csv 328 Bytes File
Fig3d_TAGContent7Weeks.csv 326 Bytes File
Fig4_C14Degradation.csv 383 Bytes File
Fig5b_ShootBiomass.csv 339 Bytes File
Fig5c_TAGContent2week.csv 337 Bytes File
Fig5d_TAGContent7week.csv 336 Bytes File
Fig6a_SeedOilDW.csv 531 Bytes File
Fig6b_SeedYieldPerPlant.csv 545 Bytes File
Fig6c_SeedOilPerPlant.csv 473 Bytes File
FigS10a_SeedProtein.csv 891 Bytes File
FigS10b_Sucrose.csv 167 Bytes File
FigS10c_GlucoseFructoseFreeAA.csv 326 Bytes File
FigS1b_ATPContent.csv 311 Bytes File
FigS2a_SeedOil.csv 365 Bytes File
FigS2b_Weight100Seeds.csv 222 Bytes File
FigS3a_FASeeds.csv 1.48 KB File
FigS3b_FALeaves.csv 1.76 KB File
FigS4a_TAGContentShoots.csv 293 Bytes File
FigS4b_LipidContentShoots.csv 294 Bytes File
FigS4c_TAGContentRoots.csv 292 Bytes File
FigS4d_LipidContentRoots.csv 295 Bytes File
FigS5a_TAGContentRosette4Week.csv 297 Bytes File
FigS5b_TAGContentRosette7Week.csv 316 Bytes File
FigS5c_FA2Week.csv 905 Bytes File
FigS6_TotalLipids.csv 469 Bytes File
FigS7a_TAGRosette4Week.csv 354 Bytes File
FigS7b_TAGRosette7Week.csv 332 Bytes File
FigS7c_FA2Week.csv 909 Bytes File
FigS8a_TAGbenth.csv 441 Bytes File
FigS8b_FAbenth.csv 898 Bytes File
FigS9a_SeedOil.csv 544 Bytes File
FigS9b_SeedWeight.csv 323 Bytes File
FigS9c_FASeedOil.csv 2.89 KB File