Metabolic Engineering of the Oleaginous Yeast Yarrowia lipolytica PO1f for Production of Erythritol from Glycerol

Jagtap, Sujit Sadashiv; Bedekar, Ashwini Ashok; Singh, Vijay; Jin, Yong-Su; Rao, Christopher V.

doi:10.13012/B2IDB-4732426_V1

Illinois Data Bank - Dataset

Metabolic Engineering of the Oleaginous Yeast Yarrowia lipolytica PO1f for Production of Erythritol from Glycerol

Cite this dataset:

Jagtap, Sujit Sadashiv; Bedekar, Ashwini Ashok; Singh, Vijay; Jin, Yong-Su; Rao, Christopher V. (2025): Metabolic Engineering of the Oleaginous Yeast Yarrowia lipolytica PO1f for Production of Erythritol from Glycerol. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-4732426_V1

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Dataset Description	Yarrowia lipolytica was found natively to produce erythritol, mannitol, and arabitol during growth on glucose, fructose, mannose, and glycerol. Osmotic stress is known to increase sugar alcohol production, and was found to significantly increase erythritol production during growth on glycerol. To better understand erythritol production from glycerol, since it was the most promising sugar alcohol, we measured the expression of key genes and intracellular metabolites. Osmotic stress increased the expression of several key genes in the glycerol catabolic pathway and the pentose phosphate pathway. Analysis of intracellular metabolites revealed that amino acids, sugar alcohols, and polyamines are produced at higher levels in response to osmotic stress. Heterologous overexpression of the sugar alcohol phosphatase increased erythritol production and glycerol utilization in Y. lipolytica. We further increased erythritol production by increasing the expression of native glycerol kinase (GK), and transketolase (TKL). These data show the growth and titers produced.
Subject	Life Sciences
Keywords	Conversion;Genome Engineering
License	CC BY
Funder	U.S. Department of Energy (DOE)-Grant:DE-SC0018420
Corresponding Creator	Christopher V. Rao
Related Materials (1) Article Jagtap S.S., Bedekar, A.A., Singh V., Jin, Y.S., Rao, C.V. Sept. 25, 2021. “Metabolic Engineering of the Oleaginous Yeast Yarrowia lipolytica PO1f for Production of Erythritol from Glycerol.” Biotechnology for Biofuels 14,188. DOI: 10.1186/s13068-021-02039-0.

Versions

Version	DOI	Comment	Publication Date
1	10.13012/B2IDB-4732426_V1		2025-10-07

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README.txt 9.02 KB File
Fig10A_PO1f-ErythritolProductionFedBatch.csv 1.13 KB File
Fig10B_PO1f_PYP_GK_TKL_ErythritolProductionFedBatch.csv 1.09 KB File
Fig1A_GlucoseGrowthPO1f.csv 635 Bytes File
Fig1B_FructoseGrowthPO1f.csv 600 Bytes File
Fig1C_MannoseGrowthPO1f.csv 579 Bytes File
Fig1D_GlycerolGrowthPO1f.csv 648 Bytes File
Fig2A_GlucoseGrowthPO1fOsmoticStress.csv 650 Bytes File
Fig2B_FructoseGrowthPO1fOsmoticStress.csv 617 Bytes File
Fig2C_MannoseGrowthPO1fOsmoticStress.csv 606 Bytes File
Fig2D_GlycerolGrowthPO1fOsmoticStress.csv 666 Bytes File
Fig3A_CN5RationErythritolProduction.csv 620 Bytes File
Fig3B_CN15RationErythritolProduction.csv 599 Bytes File
Fig3C_CN30RationErythritolProduction.csv 618 Bytes File
Fig3D_CN60RationErythritolProduction.csv 601 Bytes File
Fig4_ExpressionProfilesGlycerol.csv 661 Bytes File
Fig5_GrowthMetaboliteConcentration.csv 4.89 KB File
Fig6A_PO1f-ErythritolProduction.csv 655 Bytes File
Fig6B_PO1f_PYP_ErythritolProduction.csv 623 Bytes File
Fig6C_PO1f_GK_ErythritolProduction.csv 637 Bytes File
Fig6D_PO1f_TKL_ErythritolProduction.csv 638 Bytes File
Fig7A_PO1f_PYP_GK_ErythritolProduction.csv 725 Bytes File
Fig7B_PO1f_PYP_TKL_ErythritolProduction.csv.csv 662 Bytes File
Fig7C_PO1f_PYP_GK_ErythritolProduction.csv 709 Bytes File
Fig8_ErythritolTitersProductivities.csv 274 Bytes File
Fig9A_PO1f-ErythritolProductionCrudeGlycerol.csv 699 Bytes File
Fig9B_PO1f_PYP_GK_TKL_ErythritolProductionCrudeGlycerol.csv 724 Bytes File
FigS10_IntracellularAccumulationErythritol.csv 464 Bytes File
FigS7A_GlucoseGrowthPO1fYPMedium.csv 508 Bytes File
FigS7B_FructoseGrowthPO1fYPMedium.csv 472 Bytes File
FigS7C_MannoseGrowthPO1fYPMedium.csv 457 Bytes File
FigS7D_GlycerolGrowthPO1fYPMedium.csv 534 Bytes File
FigS8A_GlucoseGrowthPO1fOsmotic stress.csv 524 Bytes File
FigS8B_FructoseGrowthPO1fOsmotic stress.csv 512 Bytes File
FigS8C_MannoseGrowthPO1fOsmotic stress.csv 523 Bytes File
FigS8D_GlycerolGrowthPO1fOsmotic stress.csv 531 Bytes File
FigS9_FattyAcidComposition.csv 287 Bytes File
Table1_FermentationStrainData.csv 687 Bytes File
Table2_PlasmidStrainList.csv 2.11 KB File
TableS1_PCRGenes.csv 1002 Bytes File
TableS2_qPCROligonucleotides.csv 1.87 KB File
TableS3_IdentifiedMetabolites.csv.csv 2.69 KB File
TableS4_CodonOptimizedSugarAlcoholSequence.txt 791 Bytes File
TableS5_Oligonucleotides.csv 1.87 KB File