Illinois Data Bank - Dataset

Data for Noninvasive and In Situ Identification of the Phenotypes and Differentiation Stages of Individual Living Cells Entrapped Within Hydrogels
Cite this dataset:
Pastrana-Otero, Isamar; Godbole, Apurva R.; Kraft, Mary L. (2025): Data for Noninvasive and In Situ Identification of the Phenotypes and Differentiation Stages of Individual Living Cells Entrapped Within Hydrogels. University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-5669414_V1
Dataset Description

This dataset contains Raman spectra, each acquired from an individual, living, cell entrapped within a soft or stiff gelatin methacrylate hydrogel or from a cell-free region of the hydrogel sample. Spectra were acquired from the following cell types: Madin-Darby Canine Kidney cell (MDCK); Chinese hamster ovary cell (CHO-K1); transfected CHO-K1 cell that expressed the SNAP-tag and HaloTag reporter proteins fused to an organelle-specific protein (CHO-T); human monocyte-like cell (THP-1); inactive macrophage-like (M0-like); active anti-inflammatory macrophage-like (M2-like), pro/anti-inflammatory macrophage-like (M1/M2-like). These spectra are useful for identifying whether the hydrogel matrix obscures the Raman spectral signatures that are characteristic of each of these cell types.

Keywords Raman spectroscopy; 3D cell culture; single-cell spectrum; hydrogel scaffold; collagen scaffold; macrophage spectra; macrophage differentiation; THP-1 line; noninvasive phenotype identification; vibrational spectroscopy
License CC BY
Funder U.S. National Institutes of Health (NIH)-Grant:R21 HL132642
Corresponding Creator Mary L. Kraft
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Version DOI Comment Publication Date
1 10.13012/B2IDB-5669414_V1 2025-04-02

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Creator update: {"given_name"=>["Mary", "Mary L."]} 2025-04-04T20:32:15Z
Creator update: {"given_name"=>["Apurva", "Apurva R."]} 2025-04-04T20:32:15Z
Creator update: {"identifier"=>["", "0000-0003-3070-3623"]} 2025-04-04T20:32:15Z
Dataset update: {"corresponding_creator_name"=>["Mary Kraft", "Mary L. Kraft"]} 2025-04-04T20:32:15Z
Dataset update: {"keywords"=>["4.\tRaman spectroscopy; 3D cell culture; single-cell spectrum; hydrogel scaffold; collagen scaffold; macrophage spectra; macrophage differentiation; THP-1 line; noninvasive phenotype identification; vibrational spectroscopy", "Raman spectroscopy; 3D cell culture; single-cell spectrum; hydrogel scaffold; collagen scaffold; macrophage spectra; macrophage differentiation; THP-1 line; noninvasive phenotype identification; vibrational spectroscopy"]} 2025-04-04T20:29:22Z
Dataset update: {"title"=>["1.\tData for Noninvasive and In Situ Identification of the Phenotypes and Differentiation Stages of Individual Living Cells Entrapped Within Hydrogels", "Data for Noninvasive and In Situ Identification of the Phenotypes and Differentiation Stages of Individual Living Cells Entrapped Within Hydrogels"]} 2025-04-04T20:28:09Z