Data for Noninvasive and In Situ Identification of the Phenotypes and Differentiation Stages of Individual Living Cells Entrapped Within Hydrogels
Dataset Description |
This dataset contains Raman spectra, each acquired from an individual, living, cell entrapped within a soft or stiff gelatin methacrylate hydrogel or from a cell-free region of the hydrogel sample. Spectra were acquired from the following cell types: Madin-Darby Canine Kidney cell (MDCK); Chinese hamster ovary cell (CHO-K1); transfected CHO-K1 cell that expressed the SNAP-tag and HaloTag reporter proteins fused to an organelle-specific protein (CHO-T); human monocyte-like cell (THP-1); inactive macrophage-like (M0-like); active anti-inflammatory macrophage-like (M2-like), pro/anti-inflammatory macrophage-like (M1/M2-like). These spectra are useful for identifying whether the hydrogel matrix obscures the Raman spectral signatures that are characteristic of each of these cell types. |
Subject |
Life Sciences |
Keywords |
Raman spectroscopy; 3D cell culture; single-cell spectrum; hydrogel scaffold; collagen scaffold; macrophage spectra; macrophage differentiation; THP-1 line; noninvasive phenotype identification; vibrational spectroscopy |
License |
CC BY |
Funder |
U.S. National Institutes of Health (NIH)-Grant:R21 HL132642 |
Corresponding Creator |
Mary L. Kraft |
Downloaded |
301 times |
| Version | DOI | Comment | Publication Date |
|---|---|---|---|
| 1 | 10.13012/B2IDB-5669414_V1 | 2025-04-02 |
Contact the Research Data Service for help interpreting this log.
| Dataset | update: {"version_comment"=>[nil, ""], "subject"=>[nil, "Life Sciences"], "external_files_link"=>[nil, ""], "external_files_note"=>[nil, ""]} | 2025-07-16T20:32:28Z |
| Creator | update: {"given_name"=>["Mary", "Mary L."]} | 2025-04-04T20:32:15Z |
| Creator | update: {"given_name"=>["Apurva", "Apurva R."]} | 2025-04-04T20:32:15Z |
| Creator | update: {"identifier"=>["", "0000-0003-3070-3623"]} | 2025-04-04T20:32:15Z |
| Dataset | update: {"corresponding_creator_name"=>["Mary Kraft", "Mary L. Kraft"]} | 2025-04-04T20:32:15Z |
| Dataset | update: {"keywords"=>["4.\tRaman spectroscopy; 3D cell culture; single-cell spectrum; hydrogel scaffold; collagen scaffold; macrophage spectra; macrophage differentiation; THP-1 line; noninvasive phenotype identification; vibrational spectroscopy", "Raman spectroscopy; 3D cell culture; single-cell spectrum; hydrogel scaffold; collagen scaffold; macrophage spectra; macrophage differentiation; THP-1 line; noninvasive phenotype identification; vibrational spectroscopy"]} | 2025-04-04T20:29:22Z |
| Dataset | update: {"title"=>["1.\tData for Noninvasive and In Situ Identification of the Phenotypes and Differentiation Stages of Individual Living Cells Entrapped Within Hydrogels", "Data for Noninvasive and In Situ Identification of the Phenotypes and Differentiation Stages of Individual Living Cells Entrapped Within Hydrogels"]} | 2025-04-04T20:28:09Z |