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2 10.13012/B2IDB-6946735_V2 2017-03-08
1 10.13012/B2IDB-6946735_V1 2017-01-06
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update: {"peek_text"=>["GENERAL INFORMATION\n\nPI: Dr. Nathan E. Schroeder\nDepartment of Crop Sciences, University of Illinois at Urbana-Champaign.\n1102 S Goodwin Ave, IL 61801.\nEmail: nes@illinois.edu\nCo-investigator information\nSita Thapa\nDepartment of Crop Sciences, University of Illinois at Urbana-Champaign.\n1102 S Goodwin Ave, IL 61801.\nEmail: sthapa2@illinois.edu\nDate of data collection: 2015/06-2016/07\nLocation: Department of Crop Sciences, University of Illinois at Urbana-Champaign.\nTurner Hall. 1102 S Goodwin Ave, IL 61801.\nFunding source: Schlumberger Foundation and the USDA NIFA Hatch program\n\nSHARING INFORMATION\n\nLink to the publication: https://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-016-0144-7\nRecommended citation: Thapa S, Patel JA, Reutor-Carlson U, Schroeder NE. Embryogenesis in the parasitic nematode Hetrodera glycines is independent of host-derived hatching stimulation. BMC Dev Biol. 2017; 17:2.\n\nDATA AND FILE OVERVIEW\n\nMp-Fp, 1-cell, 2-cell, 3-cell, 4-cell, 5-cell, 7-cell and 8-cell: These are different cell stages of early embryo development in soybean cyst nematodes. Fig. 1 in manuscript\n\n1-cell, 2-cell, 3-cell, 4-cell, gastrula, tadpole, J1 and J2: These are different pre-hatch developmental stages of soybean cyst nematode. J1 first stage juvenile, j2 second stage juvenile. Fig. 2 in manuscript\n\n4 A: J1 molting inside the eggshell, 4B and 4D: pre-hatch J2 mechanically removed from eggshell, 4C and 4E fully formed J2. Fig. 4 in manuscript\n\n5 A B: A is pre-hatch J2 and B is a hatched J2. The stylet protractor muscle stained with Phalloidin. Fig. 5 in manuscript\n\n6A: pre-hatch J2 nerve ring stained with phalloidin; 6B: DAPI stained ventral nerve cord of hatched j2. 6C: DAPI stain ventral nerve cord of pre-hatch J2. Fig. 6 in manuscript\n\nMETHOD\nI used an upright compound microscope with a mechanized stage (Zeiss M2 AxioImager and Zen software) to get most of my data. Used fluorescence to image DAPI and Phalloidin stain nematode. Detail method is in my manuscript.\nhttps://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-016-0144-7\n\n\n\n\n", "GENERAL INFORMATION\r\n\r\nPI: Dr. Nathan E. Schroeder\r\nDepartment of Crop Sciences, University of Illinois at Urbana-Champaign.\r\n1102 S Goodwin Ave, IL 61801.\r\nEmail: nes@illinois.edu\r\nCo-investigator information\r\nSita Thapa\r\nDepartment of Crop Sciences, University of Illinois at Urbana-Champaign.\r\n1102 S Goodwin Ave, IL 61801.\r\nEmail: sthapa2@illinois.edu\r\nDate of data collection: 2015/06-2016/07\r\nLocation: Department of Crop Sciences, University of Illinois at Urbana-Champaign.\r\nTurner Hall. 1102 S Goodwin Ave, IL 61801.\r\nFunding source: Schlumberger Foundation and the USDA NIFA Hatch program\r\n\r\nSHARING INFORMATION\r\n\r\nLink to the publication: https://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-016-0144-7\r\nRecommended citation: Thapa S, Patel JA, Reutor-Carlson U, Schroeder NE. Embryogenesis in the parasitic nematode Hetrodera glycines is independent of host-derived hatching stimulation. BMC Dev Biol. 2017; 17:2.\r\n\r\nDATA AND FILE OVERVIEW\r\n\r\nMp-Fp, 1-cell, 2-cell, 3-cell, 4-cell, 5-cell, 7-cell and 8-cell: These are different cell stages of early embryo development in soybean cyst nematodes. Fig. 1 in manuscript\r\n\r\n1-cell, 2-cell, 3-cell, 4-cell, gastrula, tadpole, J1 and J2: These are different pre-hatch developmental stages of soybean cyst nematode. J1 first stage juvenile, j2 second stage juvenile. Fig. 2 in manuscript\r\n\r\n4 A: J1 molting inside the eggshell, 4B and 4D: pre-hatch J2 mechanically removed from eggshell, 4C and 4E fully formed J2. Fig. 4 in manuscript\r\n\r\n5 A B: A is pre-hatch J2 and B is a hatched J2. The stylet protractor muscle stained with Phalloidin. Fig. 5 in manuscript\r\n\r\n6A: pre-hatch J2 nerve ring stained with phalloidin; 6B: DAPI stained ventral nerve cord of hatched j2. 6C: DAPI stain ventral nerve cord of pre-hatch J2. Fig. 6 in manuscript\r\n\r\nMETHOD\r\nI used an upright compound microscope with a mechanized stage (Zeiss M2 AxioImager and Zen software) to get most of my data. Used fluorescence to image DAPI and Phalloidin stain nematode. Detail method is in my manuscript.\r\nhttps://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-016-0144-7\r\n\r\n\r\n\r\n\r\n"]} 2018-10-23T20:50:28Z
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