These data files were used for phylogenomic analyses of Darnini and related Membracidae (Hemiptera: Auchenorrhyncha) in the referenced article by Gonzalez-Mozo et al. The "mem_50p_alignment.fasta" file contains the aligned, concatenated nucleotide sequence data for 52 species and 494 genetic loci included in the phylogenetic analyses ("N" indicates missing data and "-" indicates an alignment gap). The file "Table 1.rtf" lists the included species, country of origin and genbank accession number. Species newly sequenced for this study have a Sample ID with prefix "DAR"; previously sequenced species for which data were downloaded from genbank have "NCBI" indicated in the same column of the table. The file "partition_def_50p.txt" lists the 494 genetic loci included in the alignment with their exact positions indicated by the range of numbers given at the end of each line (e.g., locus "uce-1" occupies positions 1-280 in the alignment).