Illinois Data Bank - Dataset

Alternative approaches for uncovering phytoplasma biodiversity and vector associations
Cite this dataset:
Trivellone, Valeria; Cao, Yanghui; Dietrich, Christopher (2022): Alternative approaches for uncovering phytoplasma biodiversity and vector associations. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-9804959_V1
Dataset Description

This dataset contains nucleotide sequences of 16S rRNA gene from phytoplasmas and other bacteria detected in phloem-feeding insects (Hemiptera, Auchenorrhyncha). The datasets were used to compare traditional Sanger sequencing with a next-generation sequencing method, Anchored Hybrid Enrichment (AHE) for detecting and characterizing phytoplasmas in insect DNA samples.

The file “Trivellone_etal_SangerSequencing.fas”, comprising 1397 positions (the longest sequence), includes 35 not aligned bacterial 16S rRNA sequences (16 phytoplasmas and 19 other bacterial strains) yielded using Sanger sequencing.
The file “Trivellone_etal_AHEmethod1.fas” includes 34 not aligned bacterial 16S rRNA sequences (28 phytoplasmas and 6 other bacterial strains) and it contains 1530 positions (the longest sequence). Each sequence was assembled using assembled based on ABySS v2.1.0 pipeline.
The file “Trivellone_etal_AHEmethod2.fas” includes 31 not aligned bacterial 16S rRNA sequences (27 phytoplasmas and 4 other bacterial strains) and it contains 1530 positions (the longest sequence). Each sequence was assembled based on the HybPiper v2.0.1 pipeline .
Additional details in the "read_me_trivellone.txt" file attached below.
Subject Life Sciences
Keywords anchored hybrid enrichment; biodiversity, biorepository; nested PCR; Sanger sequencing
License CC0
Funder U.S. National Science Foundation (NSF)-Grant:DEB 16-39601
Corresponding Creator Valeria Trivellone
Downloaded 182 times
Version DOI Comment Publication Date
1 10.13012/B2IDB-9804959_V1 2022-06-10

Open in Globus File Manager what's this?
35.6 KB File
42.7 KB File
42 KB File
2.9 KB File

Contact the Research Data Service for help interpreting this log.

RelatedMaterial create: {"material_type"=>"Article", "availability"=>nil, "link"=>"https://doi.org/10.3390/biology11070977", "uri"=>"10.3390/biology11070977", "uri_type"=>"DOI", "citation"=>"Trivellone, Valeria, Yanghui Cao, and Christopher H. Dietrich. 2022. \"Comparison of Traditional and Next-Generation Approaches for Uncovering Phytoplasma Diversity, with Discovery of New Groups, Subgroups and Potential Vectors\" Biology 11, no. 7: 977. https://doi.org/10.3390/biology11070977", "dataset_id"=>2276, "selected_type"=>"Article", "datacite_list"=>"IsSupplementTo"} 2022-07-05T15:17:22Z
RelatedMaterial update: {"link"=>["", "https://doi.org/10.20944/preprints202206.0122.v1"], "uri"=>[nil, "10.20944/preprints202206.0122.v1"], "uri_type"=>[nil, "DOI"], "citation"=>["Trivellone V., Cao Y., Dietrich CH. Alternative approaches for uncovering phytoplasma biodiversity and vector associations", "Trivellone, V.; Cao, Y.; Dietrich, C.H. Alternative Approaches for Uncovering Phytoplasma Biodiversity and Vector Associations. Preprints 2022, 2022060122 (doi: 10.20944/preprints202206.0122.v1)."], "datacite_list"=>[nil, "IsSupplementTo"]} 2022-06-15T15:45:25Z
Dataset update: {"version_comment"=>[nil, ""], "subject"=>[nil, "Life Sciences"]} 2022-06-15T15:45:25Z