Alternative approaches for uncovering phytoplasma biodiversity and vector associations
Dataset Description |
This dataset contains nucleotide sequences of 16S rRNA gene from phytoplasmas and other bacteria detected in phloem-feeding insects (Hemiptera, Auchenorrhyncha). The datasets were used to compare traditional Sanger sequencing with a next-generation sequencing method, Anchored Hybrid Enrichment (AHE) for detecting and characterizing phytoplasmas in insect DNA samples. The file “Trivellone_etal_SangerSequencing.fas”, comprising 1397 positions (the longest sequence), includes 35 not aligned bacterial 16S rRNA sequences (16 phytoplasmas and 19 other bacterial strains) yielded using Sanger sequencing.
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Subject |
Life Sciences |
Keywords |
anchored hybrid enrichment; biodiversity, biorepository; nested PCR; Sanger sequencing |
License |
CC0 |
Funder |
U.S. National Science Foundation (NSF)-Grant:DEB 16-39601 |
Corresponding Creator |
Valeria Trivellone |
Downloaded |
361 times |
| Version | DOI | Comment | Publication Date |
|---|---|---|---|
| 1 | 10.13012/B2IDB-9804959_V1 | 2022-06-10 |
Contact the Research Data Service for help interpreting this log.
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| RelatedMaterial | update: {"link"=>["", "https://doi.org/10.20944/preprints202206.0122.v1"], "uri"=>[nil, "10.20944/preprints202206.0122.v1"], "uri_type"=>[nil, "DOI"], "citation"=>["Trivellone V., Cao Y., Dietrich CH. Alternative approaches for uncovering phytoplasma biodiversity and vector associations", "Trivellone, V.; Cao, Y.; Dietrich, C.H. Alternative Approaches for Uncovering Phytoplasma Biodiversity and Vector Associations. Preprints 2022, 2022060122 (doi: 10.20944/preprints202206.0122.v1)."], "datacite_list"=>[nil, "IsSupplementTo"]} | 2022-06-15T15:45:25Z |
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