Dataset Search Results

This data set explores the effect of the cyanobacterial gene ictB on photosynthesis in sorghum, under both normal greenhouse growing temperatures (32 C / 25 C) and during and after an 8 day chilling stress (10 C / 5 C). IctB is a cyanobacterial gene of unknown function, which was initially thought to be involved in inorganic carbon transport into cells. While ictB is known now not to be an independently active carbon transporter in its own right, it may play a role in passive diffusion of metabolites. This transgene was introduced into sorghum by the lab of Thomas Clemente, through Agrobacterium mediated transformation, alone and in combination with the tomato sedoheptulose-1,7-bisphosphatase (SBPase) gene. Eleven events (six double construct and five single construct ictB) were involved in this study. SBPase was included because some previous experiments in C3 species and some previous modeling work, as well as its position at a metabolic branch point, indicates it plays a role as a control point for photosynthesis. A chilling treatment was included because chilling is one of the most serious ecological factors limiting the range of C4 species. Data includes gene expression, metabolomics (at normal growing temperature), SBPase enzyme activity, biomass and photosynthetic traits at both warm temperature and during and after chilling stress. ----------------- EXPLANATORY NOTES FOR ICTB/SBPASE SORGHUM MANUSCRIPT Data are organized into 10 worksheets, representing an expected 10 tables that will serve a supplementary role in the final publication. These include data on gene expression, metabolomics (at normal growing temperature), SBPase enzyme activity, biomass and photosynthetic traits at both warm temperature and during and after chilling stress. <i><b>Tables are as follows:</i></b> 1. Event_Code: for Table S1. Event codes for events and constructs. Two constructs were generated for this study, and numerous transgenic “events” (i.e. independent transformations) were carried out for each construct. A construct represents the actual vector which was introduced into the plants (complete with promoter, gene of interest, marker gene, etc.) while an event represents a single successful introduction of the transgene. Events are uniquely labeled with letter and number strings but also with a four-digit number for ease of reference, this table explains which event corresponds to each four-digit number. 2. Photosynthetic_Data: for Table S2. Photosynthetic data at greenhouse growing temperature, for ictB single construct, ictB/SBPase double construct, and wild type lines. Five ictB and six ictB/SBPase events were included. Greenhouse growing temperature was approximately 32 °C and 25 °C night. Photosynthetic parameters were measured using a Licor 6400-XT, and included parameters related to carbon dioxide uptake, water loss, and chlorophyll fluorescence. 3. Chilling_Treatment: for Table S3. Photosynthetic response to chilling treatment, for ictB single construct, and wild type lines. Four ictB events were included. Chilling treatment lasted approximately 8 days and began either 3.5 or 5.5 weeks after transplanting the plants (chilling was done in two batches). Chilling treatment involved temperatures of 10 °C day / 7 °C night in growth chambers. Photosynthetic parameters were measured at several time points during and after the chilling treatment, were measured using a Licor 6400-XT, and included parameters related to carbon dioxide uptake, water loss, and chlorophyll fluorescence. 4. SBPase_Activity: for Table S4. SBPase activity in double construct plants. These data measure in vitro substrate-saturated activity of SBPase in desalted extracts from leaf tissues, at 25 °C. Units are micromoles of SBP processed per second per m2 of leaf tissue. Five ictB/SBPase events were included. 5. 2014_gene_exp: for Table S5. Gene expression in 2014 experiment (units of cycle times). These data measure cycle times to threshold, relative to reference genes, for expression of ictB and SBPase. Six ictB single construct events and five ictB/SBPase double construct events were included. Cycle times to threshold relative to reference genes (ΔCT) are inversely related to number of transcripts relative to reference genes, as follows: ΔCT = -log2([NictB]/[Nreference])/[1 + log2b] where b = efficiency of replication. 6. 2016_gene_exp: for Table S5. Gene expression in 2016 experiment (units of cycle times). These data measure cycle times to threshold, relative to reference genes, for expression of ictB and SBPase. Six ictB single construct events and five ictB/SBPase double construct events were included. Cycle times to threshold relative to reference genes (ΔCT) are inversely related to number of transcripts relative to reference genes, as follows: ΔCT = -log2([NictB]/[Nreference])/[1 + log2b] where b = efficiency of replication. 7. Metabolites: for Table S7. Levels of 267 metabolites in leaf tissue. Four ictB single construct events and four ictB/SBPase double construct events were included in these analyses. Metabolites were measured in methanol-extracted samples, either by liquid chromatography / mass spectrometry or by gas chromatography / mass spectrometry, and were compared between events on a relative basis. As quantification was relative to wild type rather than on an absolute basis, no units are included. 8. Metabolite_F_values: for Table S8. F values for effects of ictB, SBPase (in cases where the model was better with a SBPase effect) and event. These analyses are done for each metabolite included in Table S7, and show effects of the explanatory variables ictB, SBPase, and individual event. 9. Biomass_2020: for Table S9. Biomass and grain yield at harvest, for ictB, ictB/SBPase and wild type sorghum plants in spring 2020. Four ictb/SBPase double construct and four ictB single construct events were included. 10. Biomass_2017: for Table S10. Biomass and grain yield at harvest, in chilled and non-chilled sorghum plants containing the ictB transgene (along with wild type controls) in fall 2017. Four ictB single construct events were included. Chilling treatment involved temperatures of 10 °C day / 7 °C night in growth chambers. <i><b>All the variables in the file are explained as below:</i></b> o Type (IctB-SBPase and IctB). This refers to whether a plant is wild type, single construct (contains only the ictB transgene) or double construct (contains both the ictB and SBPase transgenes). o Code: these codes are shorter labels to refer to each transgene event for the sake of convenience. o Alternate_Code: these codes are shorter labels to refer to each transgene event for the sake of convenience. o Event Number: these are unique labels for each transgenic events. o Construct Number: these are labels for each transgenic construct (either the ictB single construct or the ictB/SBPase double construct). o year (i): this refers to the year in which the study was conducted (2014, 2016, 2017, or 2020) o transgene or Transgenic: whether the transgene was present o construct or Type : whether the ictB or the ictB/SBPase construct was present (double, single, wildtype): o temp: leaf temperature during the measurement o A: carbon assimilation rate, in μmol m-2 s-1 o gs: stomatal conductance, in mol m-2 s-1 o CI: intercellular carbon dioxide concentration, in parts per million or μL L-1 o fvfm:FV’/FM’ (maximal potential photosystem II quantum yield under light adapted conditions), dimensionless ratio o phipsill: ΦPSII (maximal potential photosystem II quantum yield under light adapted conditions), dimensionless ratio o qP: photochemical quenching, i.e. ratio of ΦPSII to FV’/FM’ , dimensionless ratio o iwue: intrinsic water use efficiency, i.e. ratio of carbon assimilation rate to stomatal conductance, in units of μmol mol-1 o event: individual transgenic / transformation event o Vmax: substrate-saturated in vitro activity of the SBPase enzyme, in μmol m-2 s-1 o ID: identification number of sample o ΔCT1: difference in cycle times to threshold during gene expression (quantitative PCR) assay, between ictB and the reference gene GAPDH, in units of cycles o ΔCT2: cycle times to threshold during gene expression (quantitative PCR) assay, between SBPase and the reference gene GAPDH, in units of cycles o GAPDH: cycle times to threshold for the reference gene GAPDH (glyceraldehyde phosphate dehydrogenase) o IctB: cycle times to threshold for the gene of interest ictB o SBPase: cycle times to threshold for the gene of interest SBPase o v1 to v267 represent individual metabolite (see the heading immediately above the labels v1, v2, etc.). Variables v268-v272 refer to total (summed) metabolite levels for particular pathways of interest. o leaf: Leaf and stem dry biomass (in grams) o seed: Seedhead dry biomass (in grams) o biomass: Total (leaf, stem + seed head) dry biomass (in grams) o harvind: ratio of seed head dry biomass to total dry biomass o treatment (chilled and nonchilled): “Chilled” plants were grown under warm greenhouse conditions (32 °C day / 25 °C night) for 6 or 8 weeks, then switched to chilling temperatures under growth chamber conditions (10 °C / 7 °C night) for 8 days, and were then returned to greenhouse growing conditions. -----------------

These data obtained from the peer-reviewed literature and a public database depict the geographic expansion of the black-legged tick (Ixodes scapularis) and human cases of Lyme disease in the midwestern U.S. <b><i>Note</b></i>: There was an omission from the first version (V1) of the data set that required us to update the data. Specifically, we failed to include the data from the article "Caporale DA, Johnson CM, Millard BJ. 2005 Presence of Borrelia burgdorferi (Spirochaetales: Spirochaetaceae) in Southern Kettle Moraine State Forest, Wisconsin, and characterization of strain W97F51. J. Med. Entomol. 42, 457–472". In the second version (V2) of the data, this omission is corrected.

Dataset includes temperature data (local average April daily temperatures), first egg dates and reproductive output of Prothonotary Warblers breeding in southernmost Illinois, USA. Also included are arrival dates for warblers returning to breeding grounds from wintering grounds, and global temperature anomaly data for comparison with local temperatures. These data were used in the manuscript entitled "Warmer April Temperatures on Breeding Grounds Promote Earlier Nesting in a Long-Distance Migratory Bird, the Prothonotary Warbler" published in Frontiers in Ecology and Evolution. A rich text file is included with explanations of each variable in the dataset.

This dataset contains BEPAM model code and input data to replicate the outcomes for "The Economic and Environmental Costs and Benefits of the Renewable Fuel Standard". The dataset consists of: (1) The replication codes and data for the BEPAM model. The code file is named as output.gms. (BEPAM-Social cost model-ERL.zip) (2) Simulation results from the BEPAM model (BEPAM_Simulation_Results.csv) * Item (1) is in GAMS format. Item (2) is in text format.

This dataset contains the publication record of 6429 computer science researchers collected from the Microsoft Academic dataset provided through their Knowledge Service API (http://bit.ly/microsoft-data).

A 30 year record of the vegetation in sample plots in a woodland in the Chicago area. The changes in these plots over time show how ecological restoration can yield dramatic results.

Supporting information for "Urinary Phthalate Metabolite Concentrations and Hot Flashes in Pre- and Perimenopausal Women from the Midlife Women’s Health Study." This file contains tables of the results of stratified analyses of the associations of hot flash outcomes with urinary phthalates metabolites by menopause status, race/ethnicity, body mass index, and depressive status. This file also contains supplementary HPLC methods for the analysis of phthalate metabolites.

The data file contains a list of included studies with their detailed metadata, taken from Cochrane reviews which were used in a project associated with the manuscript "Evaluation of an automated probabilistic RCT Tagger applied to published Cochrane reviews".

The data file contains detailed information of the Cochrane reviews that were used in a project associated with the manuscript (working title) "Evaluation of an automated probabilistic RCT Tagger applied to published Cochrane reviews".

Data in this spreadsheet presents basic information on Cahokia, Mound 72 shell artifacts. This includes taxonomic identifications, provenience, and bead measurements. There are five tabs: 1. Raw data; 2. Disk bead measurements; 3. Columella bead measurements; 4. Data on cups and pendants; and, 5. Information on whole shell beads.

Raw gas exchange data for photosynthetic induction in 6 rice accession flag leaves. Photosynthetic induction and point measurements were made at ambient [CO2]. Two accessions (AUS 278 and IR64) were selected to screen in greater detail in which photosynthetic induction was measured at six [CO2].

This repository contains the datasets and corresponding results for the paper "MAGUS: Multiple Sequence Alignment using Graph Clustering". The Datasets.zip archive contains the ROSE, balibase, Gutell, and RNASim datasets used in our experiments. The Results.zip archive contains the outputs of running our methods against these datasets. Datasets used: ROSE: 10 simulated nucleotide model conditions from the SATe paper, each with 20 replicates, and with 1000 sequences per replicate. The ROSE datasets were originally taken from <a href="https://sites.google.com/eng.ucsd.edu/datasets/alignment/sate-i">https://sites.google.com/eng.ucsd.edu/datasets/alignment/sate-i</a> RNASim: This is a collection of simulated nucleotide datasets that were generated under a model of evolution that reflects selection due to RNA structural constraints. We sampled 20 subsets of 1000 sequences each, as well as 10 subsets of 10000 each, by randomly sampling from the original million-sequence RNASim dataset. Gutell: 16S.M, 16S.3, 16S.T, 16S.B.ALL: Four biological nucleotide datasets from the Comparative Ribosomal Website (CRW) with cleaned reference alignments from SATe. Since PASTA is restricted to datasets without sequence length heterogeneity, these were modified to remove sequences that deviate by more than 20% from the median length. The scrubbed datasets range from 740 to 24,246 sequences. The pre-screened 16S datasets were taken from <a href="https://sites.google.com/eng.ucsd.edu/datasets/alignment/16s23s">https://sites.google.com/eng.ucsd.edu/datasets/alignment/16s23s</a> BAliBASE: We use eight BAliBASE amino acid datasets used in the PASTA paper. As above, we remove outlier sequences, which leaves us with sizes ranging from 195 to 732 sequences. The pre-screened Balibase datasets were taken from <a href="https://sites.google.com/eng.ucsd.edu/datasets/alignment/pastaupp">https://sites.google.com/eng.ucsd.edu/datasets/alignment/pastaupp</a>

Data extracted from Text, Tables and Figures of publications in summarizing crop responses to Free-Air CO2 Elevation (FACE)

Data are from a long-term fire manipulation experiment in the Missouri Ozarks, USA. Data include the raw, annual ring-width increment (rwl), basal area increment (BAI), population-level annual growth resistance (Drs) and resilience (Drl) to drought, intrinsic water use efficiency values (WUEi) and oxygen isotopic composition of individual radial growth rings (δ18O) from southern red oak (Quercus falcata) and post oak (Q. stellata) trees. ---------------------- TITLE: Data for "Sixty-five years of fire manipulation reveals climate and fire interact to determine growth rates of Quercus spp." ---------------------- FILE OVERVIEW: This dataset contains four (4) CSV files as described below: Refsland_et_al_ECS20-0465_BAI.csv: annual basal area increment between 1948-2015 for trees across the fire manipulation experiment Refsland_et_al_ECS20-0465_DroughtIndices.csv: population-level drought resistance and resilience of trees during each target drought period Refsland_et_al_ECS20-0465_WUEi.csv: carbon isotope indicators of drought stress for trees across the fire manipulation experiment Refsland_et_al_ECS20-0465_d18Or.csv: oxygen isotope indicators of drought stress for trees across the fire manipulation experiment ---------------------- VARIABLE EXPLANATION: All the variables in those four files are explained as below: treeID: unique character string that identifies subject tree block: integer (1, 2) that identifies the study block plot: integer (1-12) that identifies the plot nested within each study block trt: character string (Annual, Control, Periodic) that identifies the fire treatment of a given plot species: character string (Quercus falcata, Quercus stellata) that identifies species of subject tree year: integer (1948-2015) that identifies the dated year of each tree ring rwl_mm: numerical value representing the annual tree ring-width, in mm bai_cm2: numerical value representing the annual basal area increment, in cm2 timeperiod: integer value (1953, 1964, 2007, 2012) representing the periods encompassing target dry and wet years Drs_2yr: numerical value representing the drought resistance, defined as the population-level annual growth of trees during drought years relative to pre-drought years for a given time period Drl_2yr: numerical value representing the drought resilience, defined as the population-level annual growth of trees following drought years relative to pre-drought years for a given time period stand_ba_m2ha: numerical value representing the total basal area of a given plot, in m2 per ha stand_density_stems_ha: numerical value representing the total stem density of a given plot, in stems per ha pool: numerical value (1-40) identifying the set of tree ring samples pooled for analysis. Samples were pooled by block, plot, year and species period: integer value (1953, 1964, 1980, 2007, 2012) representing the periods encompassing target dry and wet years type: character string (Dry, Wet) indicating the water availability of a given year d13C: numerical value representing the carbon isotopic composition of radial growth rings within a given sample pool, in per mil WUEi: numerical value representing the annual intrinsic water use efficiency of radial growth rings within a given sample pool d18O: numerical value representing the oxygen isotopic composition of radial growth rings within a given sample pool, in per mil

These are text files downloaded from the Web of Science for the bibliographic analyses found in Zinnen et al. (2020) in Applied Vegetation Science. They represent the papers and reference lists from six expert-based indicator systems: Floristic Quality Assessment, hemeroby, naturalness indicator values (& social behaviors), Ellenberg indicator values, grassland utilization values, and urbanity indicator values. To examine data, download VOSviewer and see instructrions from van Eck & Waltman (2019) for how to upload data. Although we used bibliographic coupling, there are a number of other interesting bibliographic analyses you can use with these data (e.g., visualizing citations between journals from this set of documents). Note: There are two caveats to note about these data and Supplements 1 & 2 associated with our paper. First, there are some overlapping papers in these text files (i.e., raw data). When added individually, the papers sum to more than the numbers we give. However, when combined VOSviewer recognizes these as repeats, and matches the numbers we list in S1 and the manuscript. Second, we labelled the downloaded papers in S2 with their respective systems. In some cases, the labels do not completely match our counts listed in S1 and raw data. This is because some of these papers use another system, but were not captured in our systematic literature search (e.g., a paper may have used hemeroby, but was not picked up by WoS, so this paper is not listed as one of the 52 hemeroby papers).

This file contains 13 unique case studies that were created for the One health: Infectious diseases course offered at the University of Illinois at Urbana-Champaign campus. The case studies are being made available as educational resources for other One health courses. Each case study is focused on a theme/topic which is associated with One health. These case studies were created using publicly available information and references have been provided for each case study.

These data are from an observational study and small experiment investigating reproductive biology and hybridization between two plants, Celastrus scandens L. and Celastrus orbiculatus Thunb. (Celastraceae). These data were collected during the 2008 growing season from the Indiana Dunes National Park (formerly Indiana Dunes National Lakeshore), just east of the municipality of Ogden Dunes, Indiana, USA. The five data files provide information on floral output of the two species, fertilization rate, fruit set rate, hybridization rate at two scales (individual flowers in both species, individual maternal plants in C. scandens), and the results of a hand-pollination experiment that exchanged pollen between the two species. There are six data files associated with this submission, five data files in comma-separated values format and one text file (‘readme.txt’) that includes detailed explanations of the data files.

This is a network of 14 systematic reviews on the salt controversy and their included studies. Each edge in the network represents an inclusion from one systematic review to an article. Systematic reviews were collected from Trinquart (Trinquart, L., Johns, D. M., & Galea, S. (2016). Why do we think we know what we know? A metaknowledge analysis of the salt controversy. International Journal of Epidemiology, 45(1), 251–260. https://doi.org/10.1093/ije/dyv184 ). <b>FILE FORMATS</b> 1) Article_list.csv - Unicode CSV 2) Article_attr.csv - Unicode CSV 3) inclusion_net_edges.csv - Unicode CSV 4) potential_inclusion_link.csv - Unicode CSV 5) systematic_review_inclusion_criteria.csv - Unicode CSV 6) Supplementary Reference List.pdf - PDF <b>ROW EXPLANATIONS</b> 1) Article_list.csv - Each row describes a systematic review or included article. 2) Article_attr.csv - Each row is the attributes of a systematic review/included article. 3) inclusion_net_edges.csv - Each row represents an inclusion from a systematic review to an article. 4) potential_inclusion_link.csv - Each row shows the available evidence base of a systematic review. 5) systematic_review_inclusion_criteria.csv - Each row is the inclusion criteria of a systematic review. 6) Supplementary Reference List.pdf - Each item is a bibliographic record of a systematic review/included paper. <b>COLUMN HEADER EXPLANATIONS</b> <b>1) Article_list.csv:</b> ID - Numeric ID of a paper paper assigned ID - ID of the paper from Trinquart et al. (2016) Type - Systematic review / primary study report Study Groupings - Groupings for related primary study reports from the same report, from Trinquart et al. (2016) (if applicable, otherwise blank) Title - Title of the paper year - Publication year of the paper Attitude - Scientific opinion about the salt controversy from Trinquart et al. (2016) Doi - DOIs of the paper. (if applicable, otherwise blank) Retracted (Y/N) - Whether the paper was retracted or withdrawn (Y). Blank if not retracted or withdrawn. <b>2) Article_attr.csv:</b> ID - Numeric ID of a paper year - Publication year Attitude - Scientific opinion about the salt controversy from Trinquart et al. (2016) Type - Systematic review/ primary study report <b>3) inclusion_net_edges.csv:</b> citing_ID - The numeric ID of a systematic review cited_ID - The numeric ID of the included articles <b>4) potential_inclusion_link.csv:</b> This data was translated from the Sankey diagram given in Trinquart et al. (2016) as Web Figure 4. Each row indicates a systematic review and each column indicates a primary study. In the matrix, "p" indicates that a given primary study had been published as of the search date of a given systematic review. <b>5)systematic_review_inclusion_criteria.csv:</b> ID - The numeric IDs of systematic reviews paper assigned ID - ID of the paper from Trinquart et al. (2016) attitude - Its scientific opinion about the salt controversy from Trinquart et al. (2016) No. of studies included - Number of articles included in the systematic review Study design - Study designs to include, per inclusion criteria population - Populations to include, per inclusion criteria Exposure/Intervention - Exposures/Interventions to include, per inclusion criteria outcome - Study outcomes required for inclusion, per inclusion criteria Language restriction - Report languages to include, per inclusion criteria follow-up period - Follow-up period required for inclusion, per inclusion criteria

This dataset contains experimental measurements used in the paper, "Ultra-sensitivity of Numerical Landscape Evolution Models to their Initial Conditions." (to be submitted). The data is taken from experimental runs in a miniature landscape model named the eXperimental Landscape Evolution (XLE) facility. In this facility, we complete five >24hr runs at 5 minute temporal resolution. Every five minutes, an planform image was capture, and a digital elevation model (DEM) was generated. For each run, images and a corresponding animation of images are documented. In addition,ASCII formatted DEMs along with color hillshade maps were generated. The hillshade map images were also made into an animation. This dataset is associated with the following publication: https://doi.org/10.1029/2019GL083305

This dataset is a snapshot of the presence and structure of entrepreneurship education in U.S. four-year colleges and universities in 2015, including co-curricular activities and related infrastructure. Public, private not-for-profit and for-profit institutions are included, as are specialized four-year institutions. The dataset provides insight into the presence of entrepreneurship education both within business units and in other units of college campuses. Entrepreneurship is defined broadly, to include small business management and related career-focused options.

Automatic and manual counts of black flies captured in Illinois.

Nest survival and Fledgling production data for Bell's Vireo and Willow Flycatcher nests.

Raw measurement data for umbilical remnants (umbilical vein, umbilical arteries and urachus) in support of Equine Veterinary Journal publication "Normal Regression of the Internal Umbilical Remnant Structures in Standardbred Foals."