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These are the results of an 8 month cohort study in two commercial dairy herds in Northwest Illinois. From each herd, 50 cows were selected at random, stratified over lactations 1 to 3. Serum from these animals was collected every two months and tested for antibodies to Bovine Leukosis Virus, Neospora caninum, and Mycobacterium avium subsp. paratuberculosis. Animals that left the herd during the study were replaced by another animal in the same herd and lactation. At the last sampling, serum neutralization assays were performed for Bovine Herpesvirus type 1 and Bovine Viral Diarrhea virus type 1 and 2. Production data before and after sampling was collected for the entire herd from PCdart.

This dataset contains all data used in the paper "Impact of genotype-calling methodologies on genome-wide association and genomic prediction in polyploids". The dataset includes genotypes and phenotypic data from two autotetraploid species Miscanthus sacchariflorus and Vaccinium corymbosum that was used used for genome wide association studies and genomic prediction and the scripts used in the analysis. In this V2, 2 files have the raw data are added: "Miscanthus_sacchariflorus_RADSeq.vcf" is the VCF file with the raw SNP calls of the Miscanthus sacchariflorus data used for genotype calling using the 6 genotype calling methods. "Blueberry_data_read_depths.RData" is the a RData file with the read depth data that was used for genotype calling in the Blueberry dataset.

This repository contains the data and computational analysis notebooks that were used in the following manuscript. For more information on the methods and contributing authors, please refer to the original manuscript. "Beyond A and B Compartments: how major nuclear locales define nuclear genome organization and function Omid Gholamalamdari et al. 2024"

During the transformation of wild-type (WT) Arabidopsis thaliana, a T-DNA containing OLEOSIN-GFP (OLE1-GFP) was inserted by happenstance within the GBSS1 gene, resulting in significant reduction in amylose and increase in leaf oil content in the transgenic line (OG). The synergistic effect on oil accumulation of combining gbss1 with the expression of OLE1-GFP was confirmed by transforming an independent gbss1 mutant (GABI_914G01) with OLE1-GFP. The resulting OLE1-GFP/gbss1 transgenic lines showed higher leaf oil content than the individual OLE1-GFP/WT or single gbss1 mutant lines. Further stacking of the lipogenic factors WRINKLED1, Diacylglycerol O-Acyltransferase (DGAT1), and Cys-OLEOSIN1 (an engineered sesame OLEOSIN1) in OG significantly elevated its oil content in mature leaves to 2.3% of dry weight, which is 15 times higher than that in WT Arabidopsis. Inducible expression of the same lipogenic factors was shown to be an effective strategy for triacylglycerol (TAG) accumulation without incurring growth, development, and yield penalties.

Table S1. Pollen types identified in the BCI and PNSL pollen rain data sets. Pollen types were identified to species when possible and assigned a life form based on descriptions provided in Croat, T.B. (1978). Taxa from BCI and PNSL were assigned a 1 if present in forest census data or a 0 if absent. The relative representation of each taxon has been provided for each extended record and by dry and wet season representation respectively. CA loadings are provided for axes 1 and 2 (Fig. 1).

The file “Fla.fasta”, comprising 10526 positions, is the concatenated amino acid alignments of 51 orthologues of 182 bacterial strains. It was used for the maximum likelihood and maximum parsimony analyses of Flavobacteriales. Bacterial species names and strains were used as the sequence names, host names of insect endosymbionts were shown in brackets. The file “16S.fasta” is the alignment of 233 bacterial 16S rRNA sequences. It contains 1455 positions and was used for the maximum likelihood analysis of flavobacterial insect endosymbionts. The names of endosymbiont strains were replaced by the name of their hosts. In addition to the species names, National Center for Biotechnology Information (NCBI) accession numbers were also indicated in the sequence names (e.g., sequence “Cicadellidae_Deltocephalinae_Macrostelini_Macrosteles_striifrons_AB795320” is the 16S rRNA of Macrosteles striifrons (Cicadellidae: Deltocephalinae: Macrostelini) with a NCBI accession number AB795320). The file “Sulcia_pep.fasta” is the concatenated amino acid alignments of 131 orthologues of “Candidatus Sulcia muelleri” (Sulcia). It contains 41970 positions and presents 101 Sulcia strains and 3 Blattabacterium strains. This file was used for the maximum likelihood analysis of Sulcia. The file “Sulcia_nucleotide.fasta” is the concatenated nucleotide alignment corresponding to the sequences in “Sulcia_pep.fasta” but also comprises the alignment of 16S rRNA. It has 127339 positions and was used for the maximum likelihood and maximum parsimony analyses of Sulcia. Individual gene alignments (16S rRNA and 131 orthologues of Sulcia and Blattabacterium) are deposited in the compressed file “individual_gene_alignments.zip”, which were used to construct gene trees for multispecies coalescent analysis. The names of Sulcia strains were replaced by the name of their hosts in “Sulcia_pep.fasta”, “Sulcia_nucleotide.fasta” and the files in “individual_gene_alignments.zip”. In all the alignment files, gaps are indicated by “-”.

Dataset associated with Jones et al. GCB-23-1273.R1 submission: Phenotypic signatures of urbanization? Resident, but not migratory, songbird eye size varies with urban-associated light pollution levels. Excel CSV file with all of the data used in analyses and file with descriptions of each column.

We conducted long-term capture-mark-recapture surveys on two isolated ornate box turtle (Terrapene ornata) populations in northern Illinois, USA. This dataset provides the capture history strings and additional demographic information used for estimating population vital rates with robust design capture-mark-recapture models. The vital rates were then used in a stage-based population projection matrix model for each population.

Raw arthroscopic scores, histologic scores, cytokine measurements, and performance data for the study cohort described in the accompanying publication.

This dataset includes image stacks, annotated counts, and ground-truth masks from two high-resolution sediment cores extracted from Laguna Pallcacocha, in El Cajas National Park, Ecuadorian Andes by Moy et al. (2002) and Hagemans et al. (2021). The first core (PAL 1999, from Moy et al. (2002)) extends through the Holocene (11,600 cal. yr. BP - present). There are a total of 900 annotated image stacks and masks in the PAL 1999 domain. The second core (PAL IV, from Hagemans et al. (2021)) captures the 20th century. There are 2986 annotated image stacks and masks in the PAL IV domain. Different microscopes and annotations tools were used to image and annotate each core and there are corresponding differences in naming conventions and file formats. Thus, we organized our data separately for the PAL 1999 and the PAL IV domains. The three letter codes used to label our pollen annotations are in the file: “Pollen_Identification_Codes.xlsx”. Both domain directories contain: • Image stacks organized by subdirectory • Annotations within each image stack directory, containing specimen identifications using a three letter code and coordinates defining bounding boxes or circles • Ground-truth distance-transform masks for each image stack The zip file "bestValModel_encoder.paramOnly.zip" is the trained pollen detection model produced from the images and annotations in this dataset. Please cite this dataset as: Feng, Jennifer T.; van den Berg, Thya; Donders, Timme H.; Kong, Shu; Puthanveetil Satheesan, Sandeep; Punyasena, Surangi W. (2025): Slide scans, annotated pollen counts, and trained pollen detection models for fossil pollen samples from Laguna Pallcacocha, El Cajas National Park, Ecuador . University of Illinois Urbana-Champaign. https://doi.org/10.13012/B2IDB-4207757_V1 Please also include citations of the original publications from which these data are taken: Feng, Jennifer T., Sandeep Puthanveetil Satheesan, Shu Kong, Timme H. Donders, and Surangi W. Punyasena. “Addressing the ‘Open World’: Detecting and Segmenting Pollen on Palynological Slides with Deep Learning.” bioRxiv, January 1, 2025. https://doi.org/10.1101/2025.01.05.631390. Feng, Jennifer T., Sandeep Puthanveetil Satheesan, Shu Kong, Timme H. Donders, and Surangi W. Punyasena. “Addressing the ‘Open World’: Detecting and Segmenting Pollen on Palynological Slides with Deep Learning.” Paleobiology, 2025 [in press]. Feng, J. T. (2023). Open-world deep learning applied to pollen detection (MS thesis, University of Illinois at Urbana-Champaign). https://hdl.handle.net/2142/120168

Raw data associated with PMID: 38925247

This is a research data for a manuscript - A Framework of Simulating Structural Sediment Perimeter Barriers using VFSMOD.

This dataset contain information for the paper "Changes in neuropeptide prohormone genes among Cetartio-dactyla livestock and wild species associated with evolution and domestication" Veterinary Sciences, MDPI. Protein sequences were predicted using GeneWise for 98 neuropeptide prohormone genes from publicly available genomes of 118 Cetartiodactyla species. All predictions (CetartiodactylaSequences2022.zip) were manually verified. Sequences were aligned within each prohormone using MAFFT (MDPImultalign2022.zip includes multiple sequence alignment of all species available for each prohormone). Phylogenetic gene trees were constructed using PhyML and the species tree was constructed using ASTRAL (MDPItree2022.zip). The data is released under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0).

Mitochondria play a key role in energy production and metabolism, making them a promising target for metabolic engineering and disease treatment. However, despite the known influence of passenger proteins on localization efficiency, only a few protein-localization tags have been characterized for mitochondrial targeting. To address this limitation, we leverage a Variational Autoencoder to design novel mitochondrial targeting sequences. In silico analysis reveals that a high fraction of the generated peptides (90.14%) are functional and possess features important for mitochondrial targeting. We characterize artificial peptides in four eukaryotic organisms and, as a proof-of-concept, demonstrate their utility in increasing 3-hydroxypropionic acid titers through pathway compartmentalization and improving 5-aminolevulinate synthase delivery by 1.62-fold and 4.76-fold, respectively. Moreover, we employ latent space interpolation to shed light on the evolutionary origins of dual-targeting sequences. Overall, our work demonstrates the potential of generative artificial intelligence for both fundamental research and practical applications in mitochondrial biology.

Table S2. Raw pollen counts and climatic data for each seasonal sampling period. Climatic data reflects the average daily conditions observed over the duration samples were collected (˚C/day, mm/day, MJ/m2/day). Lycopodium counts and counts for each pollen taxon reflect the aggregated pollen sum from four sampling heights.

This page contains the data for the publication "Regulation of growth and cell fate during tissue regeneration by the two SWI/SNF chromatin-remodeling complexes of Drosophila" published in Genetics, 2020

Video recorded by Louise Barker using a Cannon Powershot camera documents late-season combat behavior in Agkistrodon contortrix. Recorded in Beaufort County, North Carolina, 11.1 km SE of downtown Washington on 21 October 2020.

Table S3. Mean slope response for each predictive model used in the ecoinformatic analysis. Mean responses are provided for each seasonal and annual pollen data set analyzed from BCI and PNSL and are summarized by life form. Calculated p-values are provided for each model.

The dataset consists of results and various input data that are used in the GAMS model for the publication "Repeal of the Clean Power Plan: Social Cost and Distributional Implications". All the data are either excel files or in the .inc format which can be read within GAMS or Notepad. Main data sources include: agriculture, transportation and electricity data. Model details can be found in the paper and the GAMS model package.

This dataset contains MRI data and Imaris modeling analysis of CLARITY-cleared, immunostained tissue associated with a study that assessed the effects of lipid blends containing various levels of a hydrolyzed fat system on myelin development in healthy neonatal piglets. Data are from thirty-two piglets of mixed sexes across four diet treatment groups and includes a sow-fed reference group. MRI data (presented in Figure 2 of the associated article) consists of volumetric data from Voxel-Based Morphometry analysis in brain grey matter and white matter, as well as mean fractional anisotropy and mean orientation dispersion index data from Tract-Based Spatial Statistics analysis. Imaris data (presented in Figure 3 of the associated article) consists of twenty-one select output measures from 3D modeling analysis of PLP-stained prefrontal cortex tissue. All methods used for collection/generation/processing of data are described in the associated article: Louie AY, Rund LA, Komiyama-Kasai KA, Weisenberger KE, Stanke KL, Larsen RJ, Leyshon BJ, Kuchan MJ, Das T, Steelman AJ. A hydrolyzed lipid blend diet promotes myelination in neonatal piglets in a region and concentration-dependent manner. J Neurosci Res. 2023.

Sugarcane, a tropical C4 grass in the genus Saccharum (Poaceae), accounts for nearly 80% of sugar produced worldwide and is also an important feedstock for biofuel production. Generating transgenic sugarcane with predictable and stable transgene expression is critical for crop improvement. In this study, we generated a highly expressed single copy locus as landing pad for transgene stacking. Transgenic sugarcane lines with stable integration of a single copy nptII expression cassette flanked by insulators supported higher transgene expression along with reduced line to line variation when compared to single copy events without insulators by NPTII ELISA analysis. Subsequently, the nptII selectable marker gene was efficiently excised from the sugarcane genome by the FLPe/FRT site-specific recombination system to create selectable marker free plants. This study provides valuable resources for future gene stacking using site-specific recombination or genome editing tools.

Datasets used in the study, "Optimal completion of incomplete gene trees in polynomial time using OCTAL," presented at WABI 2017.

The associated data sets include information on stable isotopes from organic matter sources in high elevation lakes, the percentage of production assimilated from the different sources of organic matter, and the relationship between different metrics for trophic position and environmental variables.

Data from an a field survey at Nikko National Park in central Japan. Data contain information about deer carcass, environment of sites, and vertebrate scavenging.