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This dataset contains Raman spectra, each acquired from an individual, living, cell entrapped within a soft or stiff gelatin methacrylate hydrogel or from a cell-free region of the hydrogel sample. Spectra were acquired from the following cell types: Madin-Darby Canine Kidney cell (MDCK); Chinese hamster ovary cell (CHO-K1); transfected CHO-K1 cell that expressed the SNAP-tag and HaloTag reporter proteins fused to an organelle-specific protein (CHO-T); human monocyte-like cell (THP-1); inactive macrophage-like (M0-like); active anti-inflammatory macrophage-like (M2-like), pro/anti-inflammatory macrophage-like (M1/M2-like). These spectra are useful for identifying whether the hydrogel matrix obscures the Raman spectral signatures that are characteristic of each of these cell types.

This set of scripts accompanies the manuscript describing the R package polyRAD, which uses DNA sequence read depth to estimate allele dosage in diploids and polyploids. Using several high-confidence SNP datasets from various species, allelic read depth from a typical RAD-seq dataset was simulated, then genotypes were estimated with polyRAD and other software and compared to the true genotypes, yielding error estimates.

This is the data set for a publication titled, "Coupling carbon dioxide gas within a bubble curtain enhances its effectiveness to deter fish." The current study sought to quantify whether adding carbon dioxide gas (CO2) to a bubble curtain would enhance its efficacy to block fish. For this, a choice tank was outfitted with bubble curtains infused with either compressed air alone, or with two different concentrations of CO2 [30 or 100 mg/L]. Passage rates and position of common carp (an invasive Cyprinid) and black bullhead (a native Ictalurid) exposed to these treatments were compared. The data set consists of data from each of the experiments performed during the study.

Data consists of RNA expression, tuber mass, photosynthetic capacity and diurnal CO2 assimilation calculations, potato tuber nutrient content, photorespiratory metabolite analysis and meteorological data to support the increase in yield and thermotolerance observed in potato plants with an introduce photorespiratory bypass. Data was collected between 2019-2024 at University of Illinois at Urbana-Champaign, IL, USA.

This repository includes scripts and datasets for the paper, "Statistically consistent divide-and-conquer pipelines for phylogeny estimation using NJMerge." All data files in this repository are for analyses using the logdet distance matrix computed on the concatenated alignment. Data files for analyses using the average gene-tree internode distance matrix can be downloaded from the Illinois Data Bank (https://doi.org/10.13012/B2IDB-1424746_V1). The latest version of NJMerge can be downloaded from Github (https://github.com/ekmolloy/njmerge).<br /> <strong>List of Changes:</strong> &bull; Updated timings for NJMerge pipelines to include the time required to estimate distance matrices; this impacted files in the following folder: <strong>data.zip</strong> &bull; Replaced "Robinson-Foulds" distance with "Symmetric Difference"; this impacted files in the following folders: <strong> tools.zip; data.zip; scripts.zip</strong> &bull; Added some additional information about the java command used to run ASTRAL-III; this impacted files in the following folders: <strong>data.zip; astral64-trees.tar.gz (new)</strong>

Datasets and R scripts relating to the manuscript "Ecological characteristics and in situ genetic associations for yield-component traits of wild Miscanthus from eastern Russia" published in Annals of Botany, 10.1093/aob/mcw137. Field data, including collection locations, physical and ecological information for each location, and plant phenotypes relating to biomass are included. Genetic data in this repository include single nucleotide polymorphisms (SNPs) derived from restriction site-associated DNA sequencing (RAD-seq), as well as plastid microsatellites. A file is also included listing the DNA sequences of all RAD-seq markers generated to-date by the Sacks lab, including those from this publication.

This is the core data for RELIX, a dataset of vascular plant species presence for 353 prairie remnants in the Midwestern United States and associated dataset of prairie remnant metadata. The primary data file contains a list of the vascular plant species observed in the prairie remnants, as well as a metadata table with more information about the prairie remnant in question and the species list itself. The data was compiled from a variety of written sources, private and published, chronicling observations made between the mid-twentieth century and 2021. It also contains a supplementary data table of vascular plant species observed in at least 8 of the prairie remnants in RELIX, as well as a list of acknowledgements for the associated manuscript.

This page contains the data for the manuscript "Vacuolating cytotoxin A interactions with the host cell surface". This manuscript is currently in prep.

These data represent the raw data from the paper “The invasion of Japanese hop (Humulus japonicus) in a restored floodplain forest” published in Invasive Plant Science and Management by Annie H. Huang and Jeffrey W. Matthews.

This dataset is associated with the manuscript "Residual tau-fluvalinate, a beehive acaricide, disrupts growth and metabolism in the greater wax moth, Galleria mellonella" This dataset includes 2 Excel files: 1) raw_data_bioassay.xlsx: this file contains the raw data for waxworm bioassay. There are 2 worksheets within this file: - LC50: raw data for measuring the LC50 of Galleria mellonella (greater wax moth) in laboratory and field strains exposed to tau-fluvalinate. - RGR: Relative Growth Rate, raw data for measuring body weight of field strain of Galleria mellonella exposed to tau-fluvalinate. 2) raw-data_RT-qPCR.xlsx: this file contains raw data (Ct value) of RT-qPCR.

Data associated with the manuscript "Stable isotopes and diet metabarcoding reveal trophic overlap between native and invasive Banded Killifish (Fundulus diaphanus) subspecies." by Jordan H. Hartman, Mark A. Davis, Nicholas J. Iacaruso, Jeremy S. Tiemann, Eric R. Larson. For this project, we sampled six locations in Michigan and Illinois for Eastern and Western Banded Killifish and primary consumers. Using stable isotope analysis we found that Eastern Banded Killifish had higher variance in littoral dependence and trophic position than Western Banded Killifish, but both stable isotope and gut content metabarcoding analyses revealed an overlap in the diet composition and trophic position between the subspecies. This dataset provides the sampling locations, accession numbers for gut content metabarcoding data from the National Center for Biotechnology Information Sequence Read Archive, the assignment of each family used in the gut content metabarcoding analysis as littoral, pelagic, terrestrial, or parasite. and the raw stable isotope data from University of California Davis.

Data characterize zooplankton in Shelbyville Reservoir, Illinois, United States of America. Zooplankton were sampled with a conical zooplankton net (0.5m diameter mouth) when water was deeper than 2 m and by grab sample when water was shallower. Zooplankton samples were concentrated and subsampled with a Hensen-Stempel pipette following protocols described in Detmer et al. (2019). Zooplankton were identified to the lowest feasible taxonomic unit according to Pennak (1989) and Thorp and Covich (2001) and were enumerated in a 1 mL Sedgewick-Rafter cell. Subsamples were analyzed until at least 200 individuals were enumerated from each site.were counted across for each of the three main taxonomic groups (cladocerans, copepods, and rotifers). Given the variation in zooplankton concentrations at each site, this process often lead to far more than 200 individuals being counted (x̄ = 269, min = 200, max = 487). A summary of the sample size from each site can be found in Supplementary Table S2. Abundances were corrected for volume of water filtered. For rare taxa (< 20 individuals per sample), all individuals were measured for length. For abundant taxa, length measurements were collected on the first 20 organisms of each abundant taxon encountered in a subsample. Dry mass was calculated from equations for microcrustaceans, rotifers, and Chaoborus sp. (Rosen ,1981; Botrell et al., 1976; Dumont and Balvay, 1979).

Raw data from a survey of para-veterinary workers in Pakistan regarding knowledge, attitudes, and practices around ticks and tick-borne diseases. Between March and August 2023, we conducted a web-based survey among para-veterinarians recruited via email, text message, and face-to-face conversations.

Glucose and xylose are the major sugars present in cellulosic hydrolysates. The cellulosic sugars can be used for the production of platform chemicals. In this study, productions of lipid and ethanol by yeasts were compared for concentrated bioenergy sorghum syrup. Bioenergy sorghum was hydrothermally pretreated at 50% w/w solids in a continuous industrial reactor and sequentially mechanically refined using a burr mill to improve biomass accessibility for hydrolysis. Fed-batch enzymatic hydrolysis was conducted with 50% w/v solids loading and cellulase cocktail (50 FPU/g biomass) to achieve 230 g/L sugar concentration. Various strains of Rhodosporidium toruloides were evaluated for converting sugars into lipids, and strain Y-6987 had the highest lipid titer (9.2 g/L). The lipid titer was improved to 19.0 g/L by implementing a two-stage culture scheme, where the first stage was optimized for yeast growth and the second for lipid production. For ethanol production, the engineered Saccharomyces cerevisiae SR8ΔADH6 was used to coferment glucose and xylose. Ethanol fermentation was optimized for media nutrients (YP, YNB/urea, and urea), cellulosic sugar concentration, and sulfite conditioning to maximize the ethanol concentration from sorghum syrups. Fermentation of 70% v/v concentrated hydrolysate conditioned with sulfite produces 50.1 g/L ethanol from 141 g/L of sugars.

This dataset is part of a genome annoucement. The main folder PROKKA_results contain nine Prokka v.1.14.6 annotation files from nine Clostridium scindens genome sequences. Each file provide 12 output files including predicted protein sequences (.faa), nucleotide sequences of the predicted coding regions (.ffn), nucleotide sequence of the genome (.fna and .fsa), annotated genome in GenBank format (.gbk), steps recording performed during the annotation process (.log), error messages or warnings (.err), annotations in Sequin format (.sqn), summary of the annotations in tabular (.tbl), tab-separated values (.tsv) and plain text (.txt) formats.

The International Registry of Reproductive Pathology Database is part of pioneering work done by Dr. Kenneth McEntee to comprehensively document thousands of disease cases studies. His large and comprehensive collection of case reports and physical samples was complimented by development of the International Registry of Reproductive Pathology Database in the 1980s. The original FoxPro Database files and a migrated access version were completed by the College of Veterinary Medicine in 2016. Access CSV files were completed by the University of Illinois Library in 2017.

This dataset includes data on soil nitrous oxide fluxes, soil properties, and climate presented in the manuscript, "A conceptual model explaining spatial variation in soil nitrous oxide emissions in agricultural fields," published in Commucations Earth & Environment. Please refer to that publication for details about methodologies used to generate these data and for the experimental design.

This supporting information file contains codes related to pending publication Ge et al. Proc. Nat. Acad. Sci. USA, (revisions in review). The contents include a Mathematica code that solves the Laplace transformed equations and generates figures from the paper. A python code is included for generation of Figure 5 in the main text.

Count histories from camera traps and remotely sensed covariate data used in N-mixture modeling to assess the site use intensity of raccoons in Illinois.

Includes two files (.csv) behind all analyses and results in the paper published with the same title. <b>1) 'sites.species.counts'</b> is the raw 2018-2022 data from Angella Moorehouse (Illinois Nature Preserves Commission) including her 456 identified pollinator species and her raw counts per site (there may be a few errors of identification or naming, and there will always be name changes over time). Headers in columns F through Q correspond to the remnant-site labels in Figure 1 and Table 1 of the paper. Columns R to AB are the “nonremnant” sites, which have not been uniquely labelled since the specific sites aren't referenced anywhere in the manuscript. <b>2) 'C.scores'</b> has the 265 species assigned empirical C values (empirical.C) along with the four sets of expert C values and their confidence ranks (low, medium, high), and the Illinois/Indiana conservation ranks (S-ranks), following the methods described in the paper. Other headers in these files: - taxa.code: four-letter abbreviation for genus and specific name - genus: genus name - species: specific epithet - common.name: English name - group: general pollinator taxa group - empirical.C: empirically estimated conservatism score - expert#.C: conservatism score assigned by each of four experts - expert#.conf: expert's confidence in their conservatism score Blank cells in the site-species abundance matrix indicates species absence (or non-detection) Blank cells in C.scores.csv indicates missing S-ranks and unassigned C-scores (with associated missing confidence ranks) where experts lacked knowledge or confidence

The dataset is for a study conducted to understand genome-wide association (GWA) and genomic prediction of biomass yield and 14 yield-components traits in Miscanthus sacchariflorus. We evaluated a diversity panel with 590 accessions of M. sacchariflorus grown across four years in one subtropical and three temperate locations and genotyped with 268,109 single nucleotide polymorphisms (SNPs).

This study advances the production of potassium sorbate (KS) from triacetic acid lactone (TAL) utilizing food-grade solvents, ethanol (EtOH) and isopropyl alcohol (IPA). We have previously demonstrated the route to produce KS from TAL in tetrahydrofuran (THF) as the main solvent, but the use of THF is associated with environmental and health risks especially for food applications. The process employs a catalytic approach in food-grade solvents and includes three main steps: hydrogenation, etherification and hydrolysis, and ring-opening hydrolysis to produce KS from TAL. In the synthesis of KS from TAL, the use of IPA leads to higher yields and reduced reaction times compared to EtOH. As a result, the overall reaction time in IPA was reduced to 35.7 h, compared to 42.1 h in our previous study using THF and EtOH, while achieving a comparable KS yield of 84% from TAL. The synthesized KS exhibits a trans-2, trans-4 geometrical configuration, identical to that of commercially available KS. Through techno-economic analysis (TEA) and life cycle assessment (LCA), we estimated full-scale production of KS from sugarcane with the developed process in IPA could achieve a minimum product selling price (MPSP) of $8.27 per kg with a range of $7.06–10.16 per kg [5th–95th percentiles from 6000 Monte Carlo simulations] and a carbon intensity (CI) of 13.7 [9.6–18.6] kg CO2-eq per kg. This study highlights the synthesis of KS from TAL using food-grade solvents, demonstrating improved economic viability and environmental sustainability compared to our previous research (MPSP of $9.68 per kg [$8.47–11.45 per kg] and CI of 16.2 [12.0–21.2] kg CO2-eq per kg), as the total required reaction decreases while achieving the comparable overall yield of KS from TAL.

Nucleotide sequences from wild parsnip CYP71AJ4 (angelic in synthase. <a href ="https://www.ncbi.nlm.nih.gov/nuccore/EF191021">Genbank EF191021</a>) were obtained by Sanger sequencing. Seeds from individual plants from different populations were harvested to obtain corresponding cDNA. The cDNA was cloned and directly sequenced. Aminoacid translations were obtained using standard codon usage. Alignments of CYP71AJ4 sequences (involved in angular furanocoumarin biosynthesis) with as the reference sequence. Consistent amino acid variabilities were found between some populations. The relationship between sequencing variability and selective pressure is not yet known.