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Illinois Data Bank Dataset Search Results
Dataset Search Results
published: 2024-05-10
Dietrich, Christopher; Walden, Kimberly; Cao, Yanghui; Hernandez, Alvaro; Rendon, Gloria; Robinson, Gene; Skinner, Rachel; Stein, Jeffrey; Fields, Christopher (2024): High-quality genome assemblies for nine non-model North American insect species representing six orders (Insecta: Coleoptera, Diptera, Hemiptera, Hymenoptera, Lepidoptera, Neuroptera) . University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-0911874_V1
The data provided in this submission are the gene annotations for the Illinois EBP pilot project samples, as well as the predicted proteins for each sample in FASTA format.
keywords:
Earth Biogenome Project;genome assembly;Insecta;non-model species;sequencing;annotation
published: 2023-11-14
Gotsis, Dimitrios; Kelkar, Varun; Deshpande, Rucha; Brooks, Frank; KC, Prabhat; Myers, Kyle; Zeng, Rongping; Anastasio, Mark (2023): Data for the 2023 AAPM Grand Challenge on Deep Generative Modeling for Learning Medical Image Statistics. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-2773204_V3
This repository contains the training dataset associated with the 2023 Grand Challenge on Deep Generative Modeling for Learning Medical Image Statistics (DGM-Image Challenge), hosted by the American Association of Physicists in Medicine. This dataset contains more than 100,000 8-bit images of size 512x512. These images emulate coronal slices from anthropomorphic breast phantoms adapted from the VICTRE toolchain [1], with assigned X-ray attenuation coefficients relevant for breast computed tomography. Also included are the labels indicating the breast type. The challenge has now concluded. More information about the challenge can be found here: <a href="https://www.aapm.org/GrandChallenge/DGM-Image/">https://www.aapm.org/GrandChallenge/DGM-Image/</a>. * New in V3: we added a CSV file containing the image breast type labels and example images (PNG).
keywords:
Deep generative models; breast computed tomography
published: 2024-05-07
Edmonds, Devin (2024): Data for Furcifer minor Communal Oviposition Note. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-7511022_V1
Photographs and video of two Lesser Chameleons (Furcifer minor) nesting together at the same time near Itremo, Madagascar.
keywords:
reproductive biology; ecology; Madagascar; lizard; eggs; reptile
published: 2024-04-19
Zhang, Yue; Zhao, Helin; Huang, Siyuan; Hossain, Mohhamad Abir; van der Zande, Arend (2024): Enhancing Carrier Mobility In Monolayer MoS2 Transistors With Process Induced Strain. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-4074704_V1
Read me file for the data repository ******************************************************************************* This repository has raw data for the publication "Enhancing Carrier Mobility In Monolayer MoS2 Transistors With Process Induced Strain". We arrange the data following the figure in which it first appeared. For all electrical transfer measurement, we provide the up-sweep and down-sweep data, with voltage units in V and conductance unit in S. All Raman modes have unit of cm^-1. ******************************************************************************* How to use this dataset All data in this dataset is stored in binary Numpy array format as .npy file. To read a .npy file: use the Numpy module of the python language, and use np.load() command. Example: suppose the filename is example_data.npy. To load it into a python program, open a Jupyter notebook, or in the python program, run: import numpy as np data = np.load("example_data.npy") Then the example file is stored in the data object. *******************************************************************************
published: 2024-02-08
Edmonds, Devin; Sam Edmonds, Samina (2024): Data for Compsophis infralineatus Predation Note. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-8240276_V1
Photographs and video of the snake Compsophis infralineatus predating upon the chameleons Calumma crypticum and Calumma gastrotaenia near Mandraka, Madagascar.
keywords:
predation; reptile; diet
published: 2024-01-30
BK, Prajna (2024): Data for Effect of Interaural Electrode/Channel Mismatch on Interaural Coherence for Cochlear Implants. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-4136468_V1
This data set includes the cochlear implant (CI) electrodograms recorded in 2 different acoustic conditions using acoustic head KEMAR. It is a part of a study intended to explore the effect of interaural asymmetry on interaural coherence after CI processing.
keywords:
cochlear implant; electrodogram; KEMAR; interaural coherence
published: 2024-03-06
OKeefe, Joy; Bennett, Andrew (2024): Multiplex Metagenomic analyses of North American Bats - DADA2 outputs for Phyloseq. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-3079533_V1
These data are the result of analyses of the metagenome of North American bats, including 18s and 16s barcode genes designed to target microorganisms of the gut. These files are Phyloseq import files created by the DADA2 program. Each barcode gene is uploaded separately as the four files required to build a phyloseq object. For each barcode gene, the files include amplicon sequence variant (ASV) sequences, sequence tables (seqtab) which connect individual samples to the ASVs, tax tables (taxtab) which identify the taxa present as determined by a Bayesian RDP classifier, and rooted phylogenetic trees for the ASVs. Additionally, we have included a "sample_data" file which is necessary for sorting of samples across all four sequence analysis data sets by study and species. Some sample information which could identify the location of endangered species has been restricted. Multiple studies are represented in the data which can be accessed using standard methods in the Phyloseq program (e.g. For a study of bats, parasites, and gut microbiome dysregulation by Bennett, Suski, and OKeefe 2024 [in prep March 2024], study specific data can be accessed using the Study variable "DYSBIOMICS." File names include reference to the primer set used to generate them (18s primer sets: G3, G4, G6; 16s primer set: 341F3_806R5).
keywords:
metagenomics
published: 2023-08-03
Dalling, James William (2023): Data for Zombie leaves: novel repurposing of senescent fronds in the tree fern Cyathea rojasiana for nutrient uptake in a tropical montane forest. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-2925327_V1
This file contains the delta 15N values for leaf material collected from Cyathea rojasiana tree ferns before and after fertilization using ammonium -15N chloride solution to determine whether 15N update is possible from senescent leaves. Details of the experiment are provided in the online supplement to the published paper. Briefly, In February 2022 we selected three mature C. rojasiana individuals 1-1.5m in height that had leaves rooted in the soil and one new developing (but unexpanded) leaf. For each fern, two plastic pots (10 x 10 x 12 cm) were filled with a 50:50 mixture of washed river sand and soil from the Chorro watershed. For each pot, one senescent leaf that was rooted in the soil was carefully excavated and its roots transplanted into the pot. Pots were then fertilized by adding 30 ml of a 0.02 M 15N solution of ammonium-15N chloride (98% 15N; Sigma-Aldrich 299251; St Louis, MO) to yield a target concentration of 2 µg15N cm-3 of soil. After fertilization pots were carefully enclosed within thick plastic bags, and sealed around the senescent leaf rachis to prevent leaching any of 15N from the pot to the surrounding soil. At the time of N fertilization, pinnae of the youngest fully expanded leaf were collected from each fern. One pinna was collected from the base of the leaf and one from the distal end of the leaf. In March 2022, after 28 days the roots were removed from pots and two additional leaf pinnae sampled from each fern: one from the base and one from the distal end of the youngest (now fully expanded) leaf. Leaf samples were dried for 72 hours at 60 C and then leaf lamina tissue finely ground with a bead beater. The delta 15N for each leaf sample determined at the University of Illinois, Urbana-Champaign using a Thermo Delta V Advantage IRMS run in combination with a Costech 4010 Elemental Analyzer. Samples were run in continuous flow relative to laboratory standards that were calibrated with USGS 40, 41, and NBS 19 reference materials.
keywords:
15N; Cyathea rojasiana; N fertilization; montane forest
published: 2024-03-25
Xia, Yushu; Kwon, Hoyoung; Wander, Michelle (2024): Soil Nitrous Oxide Emissions Data for Estimating soil N2O emissions induced by organic and inorganic fertilizer inputs using a Tier-2, regression-based meta-analytic approach for U.S. agricultural lands". University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-9808669_V1
This accompanying study is published under the title "Estimating soil N2O emissions induced by organic and inorganic fertilizer inputs using a Tier-2, regression-based meta-analytic approach for U.S. agricultural lands" at Science of the Total Environment. The study is authored by Dr. Yushu Xia, Dr. Hoyoung Kwon, and Dr. Michelle Wander. The DOI for this study is <a href="https://doi.org/10.1016/j.scitotenv.2024.171930">https://doi.org/10.1016/j.scitotenv.2024.171930</a>.
keywords:
soil; nitrous oxide; agriculture; fertilizers; meta-analysis
published: 2019-02-19
Imker, Heidi (2019): Funding and Operating Organizations for Long-Lived Molecular Biology Databases. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-3993338_V1
The organizations that contribute to the longevity of 67 long-lived molecular biology databases published in Nucleic Acids Research (NAR) between 1991-2016 were identified to address two research questions 1) which organizations fund these databases? and 2) which organizations maintain these databases? Funders were determined by examining funding acknowledgements in each database's most recent NAR Database Issue update article published (prior to 2017) and organizations operating the databases were determine through review of database websites.
keywords:
databases; research infrastructure; sustainability; data sharing; molecular biology; bioinformatics; bibliometrics
published: 2019-03-22
Jones, Todd M.; Benson, Thomas J.; Ward, Michael P. (2019): Flight Ability of Juvenile Songbirds at Fledgling: Examples of Fledgling Drop Tests. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-2044905_V1
This data publication provides example video clips related to research on association among flight ability of juvenile songbirds at fledging and juvenile morphological traits (wing emergence, wing length, body condition, mass, and tarsus length. File names reflect the species dropped in each video. These videos are supplemental material for scientific publications by the authors and reflect an example subset of all videos collected form 2017-2018 as part of a larger study on the post-fledging ecology of grassland and shrubland birds in east-Central Illinois, USA. No birds were harmed/injured in the production of these videos and procedures were approved by the Illinois Institutional Animal Care and Use Committee (IACUC), protocol no. 18221. Individuals depicted in the videos have given consent for the videos to be shared (talent/model release form; <a href="https://publicaffairs.illinois.edu/resources/release/">https://publicaffairs.illinois.edu/resources/release/</a>)
keywords:
songbirds; flight ability; wing development; wing length; wing emergence; nestling development; post-fledging
published: 2023-07-10
Harmon-Threatt, Alexandra N.; Anderson, Nicholas L. (2023): Data for Bee movement between natural fragments is rare despite differences in species, patch, and matrix variables. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-4393989_V1
Bee movement between habitat patches in a naturally fragmented ecosystem depended on species, patch, and matrix variables. Using a mark-recapture methodology in the naturally fragmented Ozark glade ecosystem, we assessed the importance of bee size, nesting biology, the distance between patches (e.g., isolation), and nesting and floral resources in habitat patches and the surrounding matrix on bee movement. This dataset includes seven data files, three R code files, and a QGIS tool. Three of the data files include information collected at the study sites with regard to bees and matrix and patch characteristics. The other four data files are spatial files used to quantify the characteristics of the forest canopy between the study sites and the edge-to-edge distances between the study sites. R code in the R Markdown file recreates the analysis and data presentation for the associated publication. R script files contain processes for calculating some of the explanatory variables used in the analysis. The QGIS tool can be used as the first step to obtaining average values from a raster file where the cells are large relative to the areas of interest (AOI) that you would like to characterize. The second step is contained in one of the aforementioned R scripts. Detected effects included: Larger bees were more likely to move between patches. Bee movement was less likely as the distance between patches increased. However, relatively short distances (~50 m) inhibited movement more than our a priori expectations. Bees were unlikely to move away from home patches with abundant and diverse floral and below-ground nesting resources. When home patches were less resource-rich, bee movement depended on the characteristics of the away patch or the matrix. In these cases, bees were more likely to move to away patches with greater below-ground nesting and floral resources. Matrix habitats with more available floral and below-ground nesting resources appear to impede movement to neighboring patches, potentially because they already provide supplemental resources for bees.
keywords:
habitat fragmentation; bees; movement; mark-recapture; nesting resources; floral resources; isolation
published: 2019-05-16
Molloy, Erin K.; Warnow, Tandy (2019): Data from: Statistically consistent divide-and-conquer pipelines for phylogeny estimation using NJMerge. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-0569467_V2
This repository includes scripts and datasets for the paper, "Statistically consistent divide-and-conquer pipelines for phylogeny estimation using NJMerge." All data files in this repository are for analyses using the logdet distance matrix computed on the concatenated alignment. Data files for analyses using the average gene-tree internode distance matrix can be downloaded from the Illinois Data Bank (https://doi.org/10.13012/B2IDB-1424746_V1). The latest version of NJMerge can be downloaded from Github (https://github.com/ekmolloy/njmerge).<br /> <strong>List of Changes:</strong> • Updated timings for NJMerge pipelines to include the time required to estimate distance matrices; this impacted files in the following folder: <strong>data.zip</strong> • Replaced "Robinson-Foulds" distance with "Symmetric Difference"; this impacted files in the following folders: <strong> tools.zip; data.zip; scripts.zip</strong> • Added some additional information about the java command used to run ASTRAL-III; this impacted files in the following folders: <strong>data.zip; astral64-trees.tar.gz (new)</strong>
keywords:
divide-and-conquer; statistical consistency; species trees; incomplete lineage sorting; phylogenomics
published: 2019-05-31
Hahn, Jim (2019): Frequent pattern subject transactions from the University of Illinois Library (2016 - 2018). University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-9440404_V1
The data are provided to illustrate methods in evaluating systematic transactional data reuse in machine learning. A library account-based recommender system was developed using machine learning processing over transactional data of 383,828 transactions (or check-outs) sourced from a large multi-unit research library. The machine learning process utilized the FP-growth algorithm over the subject metadata associated with physical items that were checked-out together in the library. The purpose of this research is to evaluate the results of systematic transactional data reuse in machine learning. The analysis herein contains a large-scale network visualization of 180,441 subject association rules and corresponding node metrics.
keywords:
evaluating machine learning; network science; FP-growth; WEKA; Gephi; personalization; recommender systems
published: 2023-12-20
Xie, Yuxuan Richard; Castro, Daniel C.; Rubakhin, Stanislav S.; Trinklein, Timothy J.; Sweedler, Jonathan V.; Fan, Lam (2023): Integrative Multiscale Biochemical Mapping of the Brain via Deep-Learning-Enhanced High-Throughput Mass Spectrometry. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-9740536_V1
Important Note: the raw transient files need to be downloaded through this separate link: https://uofi.box.com/s/oagdxhea1wi8tvfij4robj0z0w8wq7j4. Once downloaded, place the file within the within the .d folder in the unzipped 20210930_ShortTransient_S3_5 folder to perform reconstruction step. The minimal datasets to run the computational pipeline MEISTER introduced in the manuscript titled "Integrative Multiscale Biochemical Mapping of the Brain via Deep-Learning-Enhanced High-Throughput Mass Spectrometry". The key steps of our computational pipeline include (1) tissue mass spectrometry imaging (MSI) reconstruction; (2) multimodal image registration and 3D reconstruction; (3) regional analysis; and (4) single-cell and tissue data integration. Detailed protocols to reproduce our results in the manuscript are provided with an example data set shared for learning the protocols. Our computational processing codes are implemented mostly in Python as well as MATLAB (for image registration).
keywords:
deep learning;mass spectrometry;single cells
published: 2024-02-21
Hartman, Jordan H; Corush, Joel B; Larson, Eric R; Tiemann, Jeremy S; Willink, Philip; Davis, Mark A (2024): Data for "Niche conservatism and spread explain hybridization and introgression between native and invasive fish". University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-6979965_V1
Data associated with the manuscript "Niche conservatism and spread explain hybridization and introgression between native and invasive fish" by Jordan H. Hartman, Joel B. Corush, Eric R. Larson, Jeremy S. Tiemann, Philip Willink, and Mark A. Davis. For this project, we combined results of ecological niche models (ENMs) and next-generation restriction site-associated DNA sequencing (RADseq) to test theories of niche conservatism and biotic resistance on the success of invasion, hybridization, and extent of introgression between native Western Banded Killifish and non-native Eastern Banded Killifish. This dataset provides the sampling locations and number of Banded Killifish in each population, accession numbers for RADseq from the National Center for Biotechnology Information Sequence Read Archive and the assignment of each Banded Killifish, the habitat associations of each population from the ENMs, and the occurrence points used to build the ENMs.
keywords:
Banded Killifish; ecological niche model; Fundulus diaphanus; hybrid swarm; invasive species; Laurentian Great Lakes
published: 2023-06-29
Pandit, Akshay; Karakoc, Deniz Berfin; Konar, Megan (2023): Data for: Spatially detailed agricultural and food trade between China and the United States. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-3649756_V1
This database provides estimates of agricultural and food commodity flows [in both tons and $US] between the US and China for the year 2017. Pairwise information is provided between US states and Chinese provinces, and US counties and Chinese provinces for 7 Standardized Classification of Transported Goods (SCTG) commodity categories. Additionally, crosswalks are provided to match Harmonized System (HS) codes and China's Multi-Regional Input Output (MRIO) commodity sectors to their corresponding SCTG commodity codes. The included SCTG commodities are: - SCTG 01: Iive animals and fish - SCTG 02: cereal grains - SCTG 03: agricultural products (except for animal feed, cereal grains, and forage products) - SCTG 04: animal feed, eggs, honey, and other products of animal origin - SCTG 05: meat, poultry, fish, seafood, and their preparations - SCTG 06: milled grain products and preparations, and bakery products - SCTG 07: other prepared foodstuffs, fats and oils For additional information, please see the related paper by Pandit et al. (2022) in Environmental Research Letters. ADD DOI WHEN RECEIVED
keywords:
Food flows; High-resolution; County-scale; Bilateral; United States; China
published: 2024-03-25
Suski, Cory; Dai, Qihong (2024): Data for "Differing physiological performance of coexisting cool- and warmwater fish species under heatwaves in the Midwestern United States". University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-1022017_V1
This is the dataset for the manuscript titled, "Differing physiological performance of coexisting cool- and warmwater fish species under heatwaves in the Midwestern United States"
keywords:
climate change; heat wave; metabolic rate; swimming; predator-prey interaction; thermal tolerance; Sander vitreus; walleye; largemouth bass; species distributions
published: 2024-01-19
Digrado, Anthony; Montes, Christopher; Baxter, Ivan; Ainsworth, Elizabeth (2024): Soybean seed quality response to eCO2 data files. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-6453957_V2
This data set is related to a SoyFACE experiment conducted in 2004, 2006, 2007, and 2008 with the soybean cultivars Loda and HS93-4118. The experiment looked at how seed elements were affected by elevated CO2 and yield. In this V2, 2 new files were added per journal requirement. Total there are 5 data files in text format within the digrado_et_al_gcb_data_V2 and 1 readme file. The name of files are listed below. Details about headers are explained in the readme.txt file. <b>1. ionomic_data.txt file</b> contains the ionomic data (mg/kg) for the two cultivars. The file contains all six technical replicates for each plot. The cultivar, year, treatment, and the plot from which the samples were collected are given for each entry. <b>2. yield_data.txt file</b> contains the yield data for the two cultivars (seed yield in kg/ha, seed yield in bu/a, Protein (%), Oil (%)). The file contains yield data for every plot. The cultivar, year, treatment, and the plot from which the samples were collected are given for each entry. <b>3. mineral_pro_oil_yield.txt file</b> contains the yield per hectare for each mineral (g/ha) along with the yield per hectare for protein and oil (t/ha). This was obtained by multiplying the seed content of each element (minerals, protein, and oil) by the total seed yield. The file contains yield data for every plots. The cultivar, year, treatment, and the plot from which the samples were collected are given for each entry. <b>4. economic_assessment.txt file</b> contains data used to assess the financial impact of altered seed oil content on soybean oil production. <b>5. meteorological_data.txt file</b> contains the meteorological data recorded by a weather station located ~ 3km from the experimental site (Willard Airport Champaign). Data covering the period between May 28 and September 24 were used for 2004; between May 25 and September 24 were used in 2006; between May 23 and September 17 in 2007; and between June 16 and October 24 in 2008.
keywords:
protein; oil; mineral; SoyFACE; nutrient; Glycine max; soybean; yield; CO2; agriculture; climate change
published: 2024-03-28
Zhang, Yue; Zhao, Helin; Huang, Siyuan; Hossain, Mohhamad Abir; van der Zande, Arend (2024): Enhancing Carrier Mobility In Monolayer MoS2 Transistors With Process induced Strain. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-7519929_V1
Read me file for the data repository ******************************************************************************* This repository has raw data for the publication "Enhancing Carrier Mobility In Monolayer MoS2 Transistors With Process Induced Strain". We arrange the data following the figure in which it first appeared. For all electrical transfer measurement, we provide the up-sweep and down-sweep data, with voltage units in V and conductance unit in S. All Raman modes have unit of cm^-1. ******************************************************************************* How to use this dataset All data in this dataset is stored in binary Numpy array format as .npy file. To read a .npy file: use the Numpy module of the python language, and use np.load() command. Example: suppose the filename is example_data.npy. To load it into a python program, open a Jupyter notebook, or in the python program, run: import numpy as np data = np.load("example_data.npy") Then the example file is stored in the data object. *******************************************************************************
published: 2016-12-13
Fraebel, David T.; Kuehn, Seppe (2016): Sequencing data for motility selection experiments. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-3958294_V1
BAM files for founding strain (MG1655-motile) as well as evolved strains from replicate motility selection experiments in low-viscosity agar plates containing either rich medium (LB) or minimal medium (M63+0.18mM galactose)
published: 2022-03-25
Shen, Chengze; Park, Minhyuk; Warnow, Tandy (2022): The 16S.B.ALL dataset in 100-HF condition. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-6604429_V1
This upload includes the 16S.B.ALL in 100-HF condition (referred to as 16S.B.ALL-100-HF) used in Experiment 3 of the WITCH paper (currently accepted in principle by the Journal of Computational Biology). 100-HF condition refers to making sequences fragmentary with an average length of 100 bp and a standard deviation of 60 bp. Additionally, we enforced that all fragmentary sequences to have lengths > 50 bp. Thus, the final average length of the fragments is slightly higher than 100 bp (~120 bp). In this case (i.e., 16S.B.ALL-100-HF), 1,000 sequences with lengths 25% around the median length are retained as "backbone sequences", while the remaining sequences are considered "query sequences" and made fragmentary using the "100-HF" procedure. Backbone sequences are aligned using MAGUS (or we extract their reference alignment). Then, the fragmentary versions of the query sequences are added back to the backbone alignment using either MAGUS+UPP or WITCH. More details of the tar.gz file are described in README.txt.
keywords:
MAGUS;UPP;Multiple Sequence Alignment;eHMMs
published: 2016-06-06
Fegley, Brent D. (2016): Datasets for modeling collaborative formation and collaborative "success". University of Illinois at Urbana-Champaign. https://doi.org/10.13012/J81Z429G
These datasets represent first-time collaborations between first and last authors (with mutually exclusive publication histories) on papers with 2 to 5 authors in years [1988,2009] in PubMed. Each record of each dataset captures aspects of the similarity, nearness, and complementarity between two authors about the paper marking the formation of their collaboration.
published: 2018-05-06
Sukenik, Shahar; Salam, Mohammed; Wang, Yuhan; Gruebele, Martin (2018): Dataset for: In-cell titration of small solutes controls protein stability and aggregation. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-4308433_V1
This deposit contains all raw data and analysis from the paper "In-cell titration of small solutes controls protein stability and aggregation". Data is collected into several types: 1) analysis*.tar.gz are the analysis scripts and the resulting data for each cell. The numbers correspond to the numbers shown in Fig.S1. (in publication) 2) scripts.tar.gz contains helper scripts to create the dataset in bash format. 3) input.tar.gz contains headers and other information that is fed into bash scripts to create the dataset. 4) All rawData*.tar.gz are tarballs of the data of cells in different solutes in .mat files readable by matlab, as follows: - Each experiment included in the publication is represented by two matlab files: (1) a calibration jump under amber illumination (_calib.mat suffix) (2) a full jump under blue illumination (FRET data) - Each file contains the following fields: coordleft - coordinates of cropped and aligned acceptor channel on the original image coordright - coordinates of cropped and aligned donor channel on the original image] dataleft - a 3d 12-bit integer matrix containing acceptor channel flourescence for each pixel and time step. Not available in _calib files dataright - a 3d 12-bit integer matrix containing donor channel flourescence for each pixel and time step. This will be mCherry in _calib files and AcGFP in data files. frame1 - original image size imgstd - cropped dimensions numFrames - number of frames in dataleft and dataright videos - a structure file containing camera data. Specifically, videos.TimeStamp includes the time from each frame.
keywords:
Live cell; FRET microscopy; osmotic challenge; intracellular titrations; protein dynamics
published: 2022-08-08
Shen, Chengze; Liu, Baqiao; Williams, Kelly P.; Warnow, Tandy (2022): Datasets for EMMA: A New Method for Computing Multiple Sequence Alignments given a Constraint Subset Alignment. University of Illinois at Urbana-Champaign. https://doi.org/10.13012/B2IDB-2567453_V1
This upload contains all datasets used in Experiment 2 of the EMMA paper (appeared in WABI 2023): Shen, Chengze, Baqiao Liu, Kelly P. Williams, and Tandy Warnow. "EMMA: A New Method for Computing Multiple Sequence Alignments given a Constraint Subset Alignment". The zip file has the following structure (presented as an example): salma_paper_datasets/ |_README.md |_10aa/ |_crw/ |_homfam/ |_aat/ | |_... |_... |_het/ |_5000M2-het/ | |_... |_5000M3-het/ ... |_rec_res/ Generally, the structure can be viewed as: [category]/[dataset]/[replicate]/[alignment files] # Categories: 1. 10aa: There are 10 small biological protein datasets within the `10aa` directory, each with just one replicate. 2. crw: There are 5 selected CRW datasets, namely 5S.3, 5S.E, 5S.T, 16S.3, and 16S.T, each with one replicate. These are the cleaned version from Shen et. al. 2022 (MAGUS+eHMM). 3. homfam: There are the 10 largest Homfam datasets, each with one replicate. 4. het: There are three newly simulated nucleotide datasets from this study, 5000M2-het, 5000M3-het, and 5000M4-het, each with 10 replicates. 5. rec\_res: It contains the Rec and Res datasets. Detailed dataset generation can be found in the supplementary materials of the paper. # Alignment files There are at most 6 `.fasta` files in each sub-directory: 1. `all.unaln.fasta`: All unaligned sequences. 2. `all.aln.fasta`: Reference alignments of all sequences. If not all sequences have reference alignments, only the sequences that have will be included. 3. `all-queries.unaln.fasta`: All unaligned query sequences. Query sequences are sequences that do not have lengths within 25% of the median length (i.e., not full-length sequences). 4. `all-queries.aln.fasta`: Reference alignments of query sequences. If not all queries have reference alignments, only the sequences that have will be included. 5. `backbone.unaln.fasta`: All unaligned backbone sequences. Backbone sequences are sequences that have lengths within 25% of the median length (i.e., full-length sequences). 6. `backbone.aln.fasta`: Reference alignments of backbone sequences. If not all backbone sequences have reference alignments, only the sequences that have will be included. >If all sequences are full-length sequences, then `all-queries.unaln.fasta` will be missing. >If fewer than two query sequences have reference alignments, then `all-queries.aln.fasta` will be missing. >If fewer than two backbone sequences have reference alignments, then `backbone.aln.fasta` will be missing. # Additional file(s) 1. `350378genomes.txt`: the file contains all 350,378 bacterial and archaeal genome names that were used by Prodigal (Hyatt et. al. 2010) to search for protein sequences.
keywords:
SALMA;MAFFT;alignment;eHMM;sequence length heterogeneity